Pentobarbital produces dissimilar changes in glucose influx and utilization in brain

T. Otsuka, L. Wei, D. Bereczki, V. Acuff, C. Patlak, J. Fenstermacher

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

The effects of pentobarbital sodium on local cerebral glucose utilization (LCGU) and 3-O-methylglucose (3-MG) influx were measured by quantitative autoradiography in 52 brain areas of control and treated rats. Pentobarbital (50 mg/kg ip) lowered LCGU to a relatively uniform rate (~35 μmol·100 g-1·min-1) in 24 of 25 forebrain areas. Among the 18 hindbrain areas, LCGU was decreased by pentobarbital by 15-55% (range 50-157 and 28-110 μmol·100 g-1·min-1 in control and treated rats, respectively). In contrast, pentobarbital lowered the 3-MG influx rate constant and permeability-surface area product by 20-30% in nearly all brain structures. The 3-MG results fit a model in which both the half-saturation constant and the maximal velocity of the glucose carrier are decreased by pentobarbital. After pentobarbital treatment, the ratio of local cerebral plasma flow (LCPF) to LCGU was the same as in controls for brain areas in which LCGU was -1·min-1 but was higher in brain areas where LCGU exceeded 35 μmol·100 g-1·min-1. Pentobarbital produced dissimilar changes in LCGU, 3-MG influx, and LCPF; these processes may thus not be closely linked during pentobarbital anesthesia.

Original languageEnglish
JournalAmerican Journal of Physiology - Regulatory Integrative and Comparative Physiology
Volume261
Issue number2 30-2
Publication statusPublished - 1991

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Pentobarbital
3-O-Methylglucose
Glucose
Brain
Rhombencephalon
Prosencephalon
Autoradiography
Permeability
Anesthesia

Keywords

  • 2-Deoxyglucose
  • 3-O-methyl-glucose
  • Blood-brain barrier
  • Glucose transporter
  • Local cerebral blood flow
  • Local cerebral glucose utilization

ASJC Scopus subject areas

  • Physiology

Cite this

Pentobarbital produces dissimilar changes in glucose influx and utilization in brain. / Otsuka, T.; Wei, L.; Bereczki, D.; Acuff, V.; Patlak, C.; Fenstermacher, J.

In: American Journal of Physiology - Regulatory Integrative and Comparative Physiology, Vol. 261, No. 2 30-2, 1991.

Research output: Contribution to journalArticle

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AU - Wei, L.

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AU - Patlak, C.

AU - Fenstermacher, J.

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