Penicillium verruculosum WA 30 a new source of cellulase

György Szakács, Kati Réczey, Pál Hernádi, Mária Dobozi

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Of fungi 110 strains were screened for extracellular cellulase production in shake flask experiments. Twelve strains produced the enzyme in significant quantity. Since the enzyme activity was assayed by different methods (liberation of reducing sugar from cotton, filter paper, carboxymethylcellulose and cellobiose), the estimation of the productivity of the strains differed according to the substrate used. The best cotton degrading activity per fermentation volume as well as per mg of secreted soluble protein was achieved by Penicillium verruculosum WA 30, a wild-type strain, for which the cellulase productivity has not yet been published. The cotton degrading (so-called C1) activity was successfully enhanced nearly threefold in medium experiments. Analyses of saccharification digests showed that glucose was the predominant product, with negligible amounts of cellobiose. The pH and temperature optima for WA 30 cellulase complex were pH 4.2 and 60°C.

Original languageEnglish
Pages (from-to)120-124
Number of pages5
JournalEuropean Journal of Applied Microbiology and Biotechnology
Volume11
Issue number2
DOIs
Publication statusPublished - Jan 1 1981

ASJC Scopus subject areas

  • Biotechnology
  • Microbiology

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