Penicillium chrysogenum glucose oxidase - A study on its antifungal effects

E. Leiter, F. Marx, T. Pusztahelyi, H. Haas, I. Pócsi

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

Aims: Purification and characterization of the high molecular mass Candida albicam-killing protein secreted by Penicillium chrysogenum. Methods and Results: The protein was purified by a combination of ultrafiltration, chromatofocusing and gel filtration. Enzymological characteristics [relative molecular mass (Mr) =155 000, subunit structure α2 with Mr,α = = 76 000, isoelectric point (pI) = 5.4] were determined using SDS-PAGE and 2D-electrophoresis. N-terminal amino acid sequencing and homology search demonstrated that the antifungal protein was the glucose oxidase (GOX) of the fungus. The enzyme was cytotoxic for a series of bacteria, yeasts and filamentous fungi. Vitamin C (1.0 mg ml-1) prevented oxidative cell injuries triggered by 0.004 U GOX in Emericella nidulans cultures but bovine liver catalase was ineffective even at a GOX:catalase activity ratio of 0.004:200 U. A secondary inhibition of growth in E. nidulans cultures by the oxygen-depleting GOX-catalase system was likely to replace the primary inhibition exerted by H2O2. Conclusions: Penicillium chrysogenum GOX possesses similar enzymological features to those described earlier for other Penicillium GOXs. Its cytotoxicity was dependent on the inherent antioxidant potential of the test micro-organisms. Significance and Impact of the Study: Penicillium chrysogenum GOX may find future applications in glucose biosensor production, the disinfection of medical implants or in the food industry as an antimicrobial and/or preservative agent.

Original languageEnglish
Pages (from-to)1201-1209
Number of pages9
JournalJournal of Applied Microbiology
Volume97
Issue number6
DOIs
Publication statusPublished - 2004

Fingerprint

Penicillium chrysogenum
glucose oxidase
Glucose Oxidase
Emericella
Catalase
catalase
Fungi
antifungal proteins
molecular weight
Proteins
fungi
biosensors
Penicillium
Food Industry
Disinfection
Protein Sequence Analysis
Isoelectric Point
Ultrafiltration
Biosensing Techniques
disinfection

Keywords

  • Antifungal effect
  • Candida albicans
  • Catalase
  • Emericella nidulans
  • Glucose oxidase
  • Oxidative stress
  • Penicillium chrysogenum
  • Vitamin C

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Applied Microbiology and Biotechnology
  • Biotechnology
  • Microbiology

Cite this

Penicillium chrysogenum glucose oxidase - A study on its antifungal effects. / Leiter, E.; Marx, F.; Pusztahelyi, T.; Haas, H.; Pócsi, I.

In: Journal of Applied Microbiology, Vol. 97, No. 6, 2004, p. 1201-1209.

Research output: Contribution to journalArticle

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