Penicillin V production by Penicillium chrysogenum in the presence of Fe3+ and in low-iron culture medium

E. Leiter, T. Emri, G. Gyémánt, I. Nagy, Imre Pócsi, G. Winkelmann, I. Pócsi

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Late-exponential-phase Penicillium chrysogenum mycelia grown in a complex medium possessed an intracellular iron concentration of 650 μmol/L (2.2 ± 0.6 μmol per g mycelial dry mass). This iron reserve was sufficient to ensure growth and antibiotic production after transferring mycelia into a defined low-iron minimal medium. Although the addition of Fe3+ to the Fe-limited cultures increased significantly the intracellular iron levels the surplus iron did not influence the production of penicillin V. Supplements of purified major P. chrysogenum siderophores (coprogen and ferrichrome) into the fermentation media did not affect the β-lactam production and intracellular iron level Neither 150 nor 300 μmol/L extracellular Fe3+ concentrations disturbed the glutathione metabolism of the fungus, and increased the oxidative stress caused by 700 mmol/L H2O2. Nevertheless, when iron was applied in the FeII oxidation state the oxidative cell injuries caused by the peroxide were significantly enhanced.

Original languageEnglish
Pages (from-to)127-132
Number of pages6
JournalFolia Microbiologica
Volume46
Issue number2
Publication statusPublished - 2001

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Penicillin V
Penicillium chrysogenum
Culture Media
Iron
Mycelium
Ferrichrome
Siderophores
Lactams
Peroxides
Fermentation
Glutathione
Oxidative Stress
Fungi
Anti-Bacterial Agents
Wounds and Injuries
Growth

ASJC Scopus subject areas

  • Microbiology

Cite this

Penicillin V production by Penicillium chrysogenum in the presence of Fe3+ and in low-iron culture medium. / Leiter, E.; Emri, T.; Gyémánt, G.; Nagy, I.; Pócsi, Imre; Winkelmann, G.; Pócsi, I.

In: Folia Microbiologica, Vol. 46, No. 2, 2001, p. 127-132.

Research output: Contribution to journalArticle

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AU - Nagy, I.

AU - Pócsi, Imre

AU - Winkelmann, G.

AU - Pócsi, I.

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