PCR technique assisting the early diagnosis of human papillomavirus a retrospective clinical study

Karoly Mensch, K. Szarka, Henrik Mensch, Adrienn Dobai, Zoltan Magyar, Mariana Pacurar, Aurel Claudiu Vartolomei, Daniela Manuc, Csaba Dobo Nagy

Research output: Contribution to journalArticle

Abstract

The number of oral cancers associated with Human Papillomavirus (HPV) infection is increasing worldwide. The purpose of this retrospective clinical trial is to investigate the relationship between genital and oral HPV infection and to observe the possibilities of polymerase chain reaction (PCR) technique and the limitations for oral HPV infection. The results of genital and oral HPV screening of 34 female patients and their partner, and 14 single female patients were processed in a private practice in Budapest between 2012 and 2015. After brush-biopsy sampling, HPV DNA identification and typing were performed using PCR technique. All in all 164 samples were typed. HPV DNA was detected in 76 cases (48.20%). HPV was detected in 55 cases from the genital samples (67%), and in 21 cases from the oral samples (25.6%). Genital HPV infection was statistically significantly higher in women (79.2% vs 22,9%, p<0.001). Gender comparison of genital HPV infection showed a statistically significant difference (79.2% for women, 50% for men, p = 0.006). In case of genital HPV infection, the oral HPV infection of the same person is more frequent. In this study, the above mentioned difference is significant for women and not significant for men. HPV16 is the HPV genotype which was identified in the highest ratio(47.2%). PCR technique is capable of the detection of oral HPV infection, but follow-up studies with higher case number, and questionnaire studies are needed to understand HPV transmission more accurately.

Original languageEnglish
Pages (from-to)2781-2787
Number of pages7
JournalRevista de Chimie
Volume69
Issue number10
Publication statusPublished - Oct 1 2018

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Polymerase chain reaction
Biopsy
DNA
Brushes
Screening
Sampling

Keywords

  • Human papillomavirus
  • Oral cancer
  • Polymerase chain reaction

ASJC Scopus subject areas

  • Chemistry(all)

Cite this

Mensch, K., Szarka, K., Mensch, H., Dobai, A., Magyar, Z., Pacurar, M., ... Nagy, C. D. (2018). PCR technique assisting the early diagnosis of human papillomavirus a retrospective clinical study. Revista de Chimie, 69(10), 2781-2787.

PCR technique assisting the early diagnosis of human papillomavirus a retrospective clinical study. / Mensch, Karoly; Szarka, K.; Mensch, Henrik; Dobai, Adrienn; Magyar, Zoltan; Pacurar, Mariana; Vartolomei, Aurel Claudiu; Manuc, Daniela; Nagy, Csaba Dobo.

In: Revista de Chimie, Vol. 69, No. 10, 01.10.2018, p. 2781-2787.

Research output: Contribution to journalArticle

Mensch, K, Szarka, K, Mensch, H, Dobai, A, Magyar, Z, Pacurar, M, Vartolomei, AC, Manuc, D & Nagy, CD 2018, 'PCR technique assisting the early diagnosis of human papillomavirus a retrospective clinical study', Revista de Chimie, vol. 69, no. 10, pp. 2781-2787.
Mensch K, Szarka K, Mensch H, Dobai A, Magyar Z, Pacurar M et al. PCR technique assisting the early diagnosis of human papillomavirus a retrospective clinical study. Revista de Chimie. 2018 Oct 1;69(10):2781-2787.
Mensch, Karoly ; Szarka, K. ; Mensch, Henrik ; Dobai, Adrienn ; Magyar, Zoltan ; Pacurar, Mariana ; Vartolomei, Aurel Claudiu ; Manuc, Daniela ; Nagy, Csaba Dobo. / PCR technique assisting the early diagnosis of human papillomavirus a retrospective clinical study. In: Revista de Chimie. 2018 ; Vol. 69, No. 10. pp. 2781-2787.
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