PCR studies on the potential sites for latency of BHV-4 in calves

L. Egyed, A. Bartha

Research output: Contribution to journalArticle

42 Citations (Scopus)


The aim of the study was to examine various tissues of experimentally infected calves for the BHV-4 genome so as to detect in which cells the virus persists during the latent phase of the infection. The presence of the bovine herpesvirus type 4 genome was detected by a nested PCR in a variety of tissues collected from two susceptible calves experimentally infected 62 days earlier. Mild clinical signs of bronchitis, an elevated body temperature for 2-3 days, and a slightly increased number of blood leukocytes were observed in both inoculated calves. BHV-4 was demonstrated in seven samples from the 12 different parts of the nervous system tested from each calf (29.1%), from the cornea, from lymph nodes near to the inoculation site, from the gallbladder and from the bone marrow. Thus a member of the predominantly lymphotropic Gammaherpesvirinae subfamily was detected in neural tissue and other organs that have never been associated with persistence.

Original languageEnglish
Pages (from-to)209-216
Number of pages8
JournalVeterinary research communications
Issue number3
Publication statusPublished - Jan 1 1998



  • Bone marrow
  • Bovine herpesvirus type 4
  • Calves
  • Conjunctiva
  • Cornea
  • Latency
  • Nervous tissue
  • PCR

ASJC Scopus subject areas

  • veterinary(all)

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