PCR detection of bovine herpesviruses from nonbovine ruminants in Hungary

Dóra Kálmán, L. Egyed

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Polymerase chain reaction (PCR) was used to test six different nonbovine ruminant species for five bovine herpesviruses including infectious bovine rhinotracheitis virus (BoHV-1), bovine herpes mammillitis virus (BoHV-2), Movar-type herpesvirus (BoHV-4), bovine herpesvirus type 5 (BoHV-5), and alcelaphine herpesvirus 1 (AlHV-1). Species tested included 56 roe deer (Capreolus capreolus), 66 red deer (Cervus elaphus), 20 fallow deer (Dama dama), 16 mouflon (Ovis musimon), 34 domestic sheep, and 44 domestic goats, which were sampled in Hungary in 2003. Tracheal and popliteal lymph nodes collected from these animals were tested for the presence of the five bovine herpesviruses using three nested (two of which were duplex) PCR assays. Three bovine herpesviruses (BoHV-1, -2, and -4) were detected, whereas no evidence of AlHV-1 or BoHV-5 was observed. Prevalence of BoHV-1 ranged from 12% to 47%, and PCR-positive results were observed in all species tested. BoHV-2 was detected from roe deer, red deer, fallow deer, mouflon, and domestic sheep, and the prevalence in these species ranged from 3% to 50%. BoHV-4 was detected in all species, with prevalence ranging from 12% to 69%. Sequenced PCR products were 99-100% identical to bovine herpesviral sequences deposited in the GenBank.

Original languageEnglish
Pages (from-to)482-488
Number of pages7
JournalJournal of Wildlife Diseases
Volume41
Issue number3
Publication statusPublished - Jul 2005

Fingerprint

Deer
Hungary
Herpesviridae
ruminant
Ruminants
Domestic Sheep
polymerase chain reaction
ruminants
deer
Bovine herpesvirus 1
Polymerase Chain Reaction
Bovine Herpesvirus 5
mouflon
cattle
Cervus dama
Capreolus capreolus
Bovine herpesvirus 2
Bovine Herpesvirus 1
Cervus elaphus
Bovine herpesvirus 4

Keywords

  • Alcelaphine herpesvirus 1
  • AlHV-1
  • BoHV-1
  • BoHV-2
  • BoHV-4
  • BoHV-5
  • Bovine herpesviruses
  • Cervids
  • Deer
  • PCR

ASJC Scopus subject areas

  • veterinary(all)

Cite this

PCR detection of bovine herpesviruses from nonbovine ruminants in Hungary. / Kálmán, Dóra; Egyed, L.

In: Journal of Wildlife Diseases, Vol. 41, No. 3, 07.2005, p. 482-488.

Research output: Contribution to journalArticle

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