Patterns of Dwarf expression and brassinosteroid accumulation in tomato reveal the importance of brassinosteroid synthesis during fruit development

Teresa Montoya, Takahito Nomura, Takao Yokota, Kerrie Farrar, Kate Harrison, Jonathan G D Jones, Tsuyoshi Kaneta, Yuji Kamiya, M. Szekeres, Gerard J. Bishop

Research output: Contribution to journalArticle

80 Citations (Scopus)

Abstract

Brassinosteroids (BRs) are essential for many physiological functions in plants, however little is known concerning where and when they are synthesized. This is especially true during flower and fruit production. To address this we have used a promoter-GUS reporter fusion and RT-PCR to determine the relative expression levels of the tomato Dwarf (D) gene that encodes a BR C-6 oxidase. In young seedlings GUS reporter activity was observed mainly in apical and root tissues undergoing expansion. In flowers GUS activity was observed in the pedicel joints and ovaries, whereas in fruits it was strongest during early seed development and was associated with the locular jelly and seeds. RT-PCR analysis showed that tissue-specific expression of Dwarf mRNA was consistent with that of the Dwarf:GUS fusion. In good correlation with the high local Dwarf activity, quantitative measurements of endogenous BRs indicated intense biosynthesis in developing tomato fruits, which were also found to contain high amounts of brassinolide. Grafting experiments showed the lack of BR transport indicating that BR action occurs at the site of synthesis.

Original languageEnglish
Pages (from-to)262-269
Number of pages8
JournalPlant Journal
Volume42
Issue number2
DOIs
Publication statusPublished - Apr 2005

Fingerprint

Brassinosteroids
brassinosteroids
Lycopersicon esculentum
fruiting
Fruit
tomatoes
synthesis
Seeds
reverse transcriptase polymerase chain reaction
Tissue Expansion
flowers
brassinolide
Polymerase Chain Reaction
tissue analysis
fruits
jellies
pedicel
grafting (plants)
seed development
Seedlings

Keywords

  • Brassinosteroids
  • Fruit
  • Grafting
  • GUS
  • Tomato Dwarf gene expression

ASJC Scopus subject areas

  • Plant Science

Cite this

Patterns of Dwarf expression and brassinosteroid accumulation in tomato reveal the importance of brassinosteroid synthesis during fruit development. / Montoya, Teresa; Nomura, Takahito; Yokota, Takao; Farrar, Kerrie; Harrison, Kate; Jones, Jonathan G D; Kaneta, Tsuyoshi; Kamiya, Yuji; Szekeres, M.; Bishop, Gerard J.

In: Plant Journal, Vol. 42, No. 2, 04.2005, p. 262-269.

Research output: Contribution to journalArticle

Montoya, Teresa ; Nomura, Takahito ; Yokota, Takao ; Farrar, Kerrie ; Harrison, Kate ; Jones, Jonathan G D ; Kaneta, Tsuyoshi ; Kamiya, Yuji ; Szekeres, M. ; Bishop, Gerard J. / Patterns of Dwarf expression and brassinosteroid accumulation in tomato reveal the importance of brassinosteroid synthesis during fruit development. In: Plant Journal. 2005 ; Vol. 42, No. 2. pp. 262-269.
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