Pathophysiological process after transient ischemia of the middle cerebral artery in the rat

H. Nishino, A. Czurkó, A. Fukuda, T. Hashitani, H. Hida, Z. Karadi, L. Lénárd

Research output: Contribution to journalArticle

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Abstract

For the understanding of pathophysiology of the cerebral ischemia, we made a transient intraluminal occlusion of the middle cerebral artery in the rat and investigated the appearance of collapsed dark neurons and the extravasation of serum proteins using argyrophil III method and immunohistochemistry. In the acute stage (minutes to 3 days), dark neurons appeared in the lateral half of the ipsilateral striatum and adjacent cortex which formed the ischemic core of this model. Dark neurons also appeared in the ipsilateral reticular thalamic nucleus, hippocampus, and amygdala. The extravasation of serum proteins, albumin, leucocyte common antigen, immunoglobulin G, complement factor C3, as well as heat shock protein 70, was observed not only in the ischemic but sometimes also in the contralateral hemisphere. Among these, the expression of IgG and C3 was most prominent in the ischemic core. In the chronic stage (1 to 3 months), the ischemic core changed into the porencephaly, and the ventrobasal nucleus of the thalamus got also involved in the necrosis. A strong microgliosis was observed in the substantia nigra pars reticulata. Data suggest, that among many mechanisms that contribute to ischemic neuronal death, the activation of immune response, due to the damage of bloodbrain barrier and the extravasation of serum proteins could promote the ischemic cell death in the brain.

Original languageEnglish
Pages (from-to)51-56
Number of pages6
JournalBrain Research Bulletin
Volume35
Issue number1
DOIs
Publication statusPublished - 1994

Fingerprint

Middle Cerebral Artery
Blood Proteins
Ischemia
Neurons
Immunoglobulin G
CD45 Antigens
Thalamic Nuclei
Complement C3
HSP70 Heat-Shock Proteins
Middle Cerebral Artery Infarction
Amygdala
Brain Ischemia
Thalamus
Serum Albumin
Hippocampus
Cell Death
Necrosis
Immunohistochemistry
Brain

Keywords

  • Argyrophilia
  • C3 Blood-brain barrier
  • Complement
  • Cortex
  • IgG
  • Ischemia
  • Middle cerebral artery
  • Serum protein
  • Striatum

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Pathophysiological process after transient ischemia of the middle cerebral artery in the rat. / Nishino, H.; Czurkó, A.; Fukuda, A.; Hashitani, T.; Hida, H.; Karadi, Z.; Lénárd, L.

In: Brain Research Bulletin, Vol. 35, No. 1, 1994, p. 51-56.

Research output: Contribution to journalArticle

Nishino, H. ; Czurkó, A. ; Fukuda, A. ; Hashitani, T. ; Hida, H. ; Karadi, Z. ; Lénárd, L. / Pathophysiological process after transient ischemia of the middle cerebral artery in the rat. In: Brain Research Bulletin. 1994 ; Vol. 35, No. 1. pp. 51-56.
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