Pathologic alterations of the outer retina in streptozotocin-induced diabetes

Anna Énzsöly, Arnold Szabó, Orsolya Kántor, Csaba Dávid, Péter Szalay, Klaudia Szabó, Ágoston Szél, János Németh, Ákos Lukáts

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

Purpose. Neurodegeneration as an early event of diabetic retinopathy preceding clinically detectable vascular alterations is a widely proven issue today. While there is evidence for the impairment of color vision and contrast sensitivity in early diabetes, suggesting deteriorated photoreceptor function, the underlying neuropathology of these functional alterations is still unknown. The aim of the present study was to investigate the effects of early diabetes on the outer retinal cells. Methods. The retinal pigment epithelium, photopigment expression, and density and morphology of photoreceptors were studied using immunocytochemistry in streptozotocininduced diabetes in two rat strains. The fine structure of photoreceptors and pigment epithelium was also investigated with transmission electron microscopy. Results. Here we found that retinal thickness was unchanged in diabetic animals and that no significant increase in the number of apoptotic cells was present. Although the density of cones expressing middle (M)- and shortwave (S)-sensitive opsins was similar in diabetic and control retinas, we detected remarkable morphologic signs of degeneration in the outer segments of diabetic rods, most M-cones, and some S-cones. A decrease in thickness and RPE65 protein immunoreactivity of the pigment epithelium were evident. Furthermore, an increased number of dual cones, coexpressing both M- and S-opsins, was detected at the peripheral retina of diabetic rats. Conclusions. Degenerative changes of photoreceptors and pigment epithelium shown here prior to apoptotic loss of photoreceptors may contribute to functional alterations reported in diabetic human patients and different animal models, thus may serve as a potential model for testing the efficacy of neuroprotective agents in diabetes.

Original languageEnglish
Pages (from-to)3686-3699
Number of pages14
JournalInvestigative Ophthalmology and Visual Science
Volume55
Issue number6
DOIs
Publication statusPublished - May 20 2014

Keywords

  • Diabetes mellitus
  • Neurodegeneration
  • Photoreceptor morphology
  • Retinal pigment epithelium

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience

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