Patchy myocardial pattern of virus sequence persistence in heart transplant recipients - Possible role of sampling error in the etiology

I. Hartyánszky, E. Tátrai, A. Lászik, M. Hubay, Z. Szelíd, G. Acsády, Z. Szabolcs, B. Merkely, F. Horkay, P. Sótonyi

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Background: The pathway from viral myocarditis to end-stage heart failure is commonly accepted, but diagnosis of virus-mediated myocardial injury remains challenging. Virus persistency in the myocardium may accelerate ventricular failure; thus, a precise diagnosis of virus persistency may prevent the development of end-stage heart failure. Methods: We performed a systematic investigation on the sampling error of viral diagnostics in heart transplant recipients: Transmural samples from 5 regions of the explanted hearts from recipients during heart transplantation were amplified using entero-, adeno-, and herpesvirus sequences and histologic examinations performed. Results: We examined 175 myocardial samples from dilated cardiomyopathy and 100 samples from 20 forensic medicine patients. Seven patients were positive for the examined viruses: 10 positive regions for adenovirus, and 1 positive region for herpes virus DNA, but none for enterovirus. A focal myocardial pattern was detected for adenovirus. Conclusion: Our results with the patchy myocardial viral persistence may explain possible false-negative results related to virus-mediated etiology among end-stage dilated cardiomyopathy patients. Therefore, repeated endomyocardal biopsies, and multiple cardiac samples are recommended to be obtained to evaluate the etiology of heart failure, thus reducing the occurrence of end-stage heart failure and decreasing the number of patients requiring heart transplantation.

Original languageEnglish
Pages (from-to)1285-1289
Number of pages5
JournalTransplantation proceedings
Volume43
Issue number4
DOIs
Publication statusPublished - May 1 2011

ASJC Scopus subject areas

  • Surgery
  • Transplantation

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