Participation of nitric oxide in the nicotine-induced relaxation of the cat lower esophageal sphincter

Nadejda Kortezova, Vera Velkova, Zlatka Mizhorkova, Gisela Bredy-Dobreva, E. Vízi, Maria Papasova

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

The participation of nitric oxide in the relaxation of the cat lower esophageal sphincter muscle strip in response to electrical field stimulation or administration of nicotine was studied. The nicotine-induced relaxation was mediated via a neuronal pathway, since it was inhibited by administration of hexamethonium or tetrodotoxin. Inhibition of nitric oxide biosynthesis by N-nitro-l-arginine decreased the relaxation induced by nicotine (50 μM) or field stimulation. With the maximal concentration of N-nitro-l-arginine (1 mM) electrical field stimulation-induced relaxation was abolished, while nicotine-induced relaxation decreased by 70%. l-Arginine (1 mM) partly restored this relaxation. Desensitization of P2x receptors by α,β methylene-adenosine 5-triphosphate (α,β-m-ATP) did not change the relaxation induced by either electrical field stimulation or administration of nicotine. It is therefore suggested that the field stimulation-induced relaxation is mediated by the release of nitric oxide, but in the nicotine-produced relaxation is only partly due to nitric oxide, other factor(s) might be also be involved.

Original languageEnglish
Pages (from-to)73-78
Number of pages6
JournalJournal of the Autonomic Nervous System
Volume50
Issue number1
DOIs
Publication statusPublished - Dec 1 1994

Fingerprint

Lower Esophageal Sphincter
Nicotine
Nitric Oxide
Cats
Electric Stimulation
Arginine
Hexamethonium
Tetrodotoxin
Adenosine Triphosphate
Muscles

Keywords

  • Lower esophageal sphincter
  • Nicotine
  • Nitric oxide
  • Relaxation

ASJC Scopus subject areas

  • Physiology
  • Clinical Neurology
  • Neuroscience(all)

Cite this

Participation of nitric oxide in the nicotine-induced relaxation of the cat lower esophageal sphincter. / Kortezova, Nadejda; Velkova, Vera; Mizhorkova, Zlatka; Bredy-Dobreva, Gisela; Vízi, E.; Papasova, Maria.

In: Journal of the Autonomic Nervous System, Vol. 50, No. 1, 01.12.1994, p. 73-78.

Research output: Contribution to journalArticle

Kortezova, Nadejda ; Velkova, Vera ; Mizhorkova, Zlatka ; Bredy-Dobreva, Gisela ; Vízi, E. ; Papasova, Maria. / Participation of nitric oxide in the nicotine-induced relaxation of the cat lower esophageal sphincter. In: Journal of the Autonomic Nervous System. 1994 ; Vol. 50, No. 1. pp. 73-78.
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