Partial cloning and localization of leptin and leptin receptor in the mammary gland of the Egyptian water buffalo

A. Sayed-Ahmed, S. Elm Elmorsy, P. Rudas, T. Bartha

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Originally an overall metabolic control was attributed to the leptin hormone, which is produced mainly by the adipose tissue. Recently, leptin gene expression was demonstrated in several additional peripheral tissues. Furthermore, several isoforms of leptin receptor were found both in the central nervous system and in the peripheral tissues. Using reverse transcription and polymerase chain reaction analysis we demonstrate that leptin is expressed both in the adipose tissue and in the lactating mammary gland tissue of Egyptian water buffalo. Our results show that, short and long isoforms of leptin receptor are expressed in buffalo mammary gland tissue. We have partially cloned the buffalo leptin and its short and long isoforms of receptor, which show a high sequence homology to previously published sequences of other mammalian species especially to that of other ruminants. Localization of leptin and its receptor mRNA transcripts, as determined by in situ hybridization procedure, revealed that leptin and its receptor transcripts are expressed specifically in the alveolar epithelial cells of the mammary gland. These morphological data support that leptin could also act as an autocrine and paracrine mediator for mammary gland metabolism and as a facilitator of alveolar epithelial cell activity during lactation.

Original languageEnglish
Pages (from-to)303-314
Number of pages12
JournalDomestic Animal Endocrinology
Volume25
Issue number3
DOIs
Publication statusPublished - Oct 2003

Fingerprint

Leptin Receptors
Buffaloes
Human Mammary Glands
Leptin
leptin
mammary glands
buffaloes
Organism Cloning
molecular cloning
Alveolar Epithelial Cells
Protein Isoforms
Adipose Tissue
receptors
adipose tissue
epithelial cells
Ruminants
Sequence Homology
Lactation
Reverse Transcription
In Situ Hybridization

Keywords

  • Buffalo
  • Lactation
  • Leptin
  • Leptin receptor
  • Mammary gland

ASJC Scopus subject areas

  • Animal Science and Zoology
  • veterinary(all)
  • Endocrinology

Cite this

Partial cloning and localization of leptin and leptin receptor in the mammary gland of the Egyptian water buffalo. / Sayed-Ahmed, A.; Elmorsy, S. Elm; Rudas, P.; Bartha, T.

In: Domestic Animal Endocrinology, Vol. 25, No. 3, 10.2003, p. 303-314.

Research output: Contribution to journalArticle

@article{56eefdbdcc3d44d29b537a9e76c36a37,
title = "Partial cloning and localization of leptin and leptin receptor in the mammary gland of the Egyptian water buffalo",
abstract = "Originally an overall metabolic control was attributed to the leptin hormone, which is produced mainly by the adipose tissue. Recently, leptin gene expression was demonstrated in several additional peripheral tissues. Furthermore, several isoforms of leptin receptor were found both in the central nervous system and in the peripheral tissues. Using reverse transcription and polymerase chain reaction analysis we demonstrate that leptin is expressed both in the adipose tissue and in the lactating mammary gland tissue of Egyptian water buffalo. Our results show that, short and long isoforms of leptin receptor are expressed in buffalo mammary gland tissue. We have partially cloned the buffalo leptin and its short and long isoforms of receptor, which show a high sequence homology to previously published sequences of other mammalian species especially to that of other ruminants. Localization of leptin and its receptor mRNA transcripts, as determined by in situ hybridization procedure, revealed that leptin and its receptor transcripts are expressed specifically in the alveolar epithelial cells of the mammary gland. These morphological data support that leptin could also act as an autocrine and paracrine mediator for mammary gland metabolism and as a facilitator of alveolar epithelial cell activity during lactation.",
keywords = "Buffalo, Lactation, Leptin, Leptin receptor, Mammary gland",
author = "A. Sayed-Ahmed and Elmorsy, {S. Elm} and P. Rudas and T. Bartha",
year = "2003",
month = "10",
doi = "10.1016/S0739-7240(03)00057-2",
language = "English",
volume = "25",
pages = "303--314",
journal = "Domestic Animal Endocrinology",
issn = "0739-7240",
publisher = "Elsevier Inc.",
number = "3",

}

TY - JOUR

T1 - Partial cloning and localization of leptin and leptin receptor in the mammary gland of the Egyptian water buffalo

AU - Sayed-Ahmed, A.

AU - Elmorsy, S. Elm

AU - Rudas, P.

AU - Bartha, T.

PY - 2003/10

Y1 - 2003/10

N2 - Originally an overall metabolic control was attributed to the leptin hormone, which is produced mainly by the adipose tissue. Recently, leptin gene expression was demonstrated in several additional peripheral tissues. Furthermore, several isoforms of leptin receptor were found both in the central nervous system and in the peripheral tissues. Using reverse transcription and polymerase chain reaction analysis we demonstrate that leptin is expressed both in the adipose tissue and in the lactating mammary gland tissue of Egyptian water buffalo. Our results show that, short and long isoforms of leptin receptor are expressed in buffalo mammary gland tissue. We have partially cloned the buffalo leptin and its short and long isoforms of receptor, which show a high sequence homology to previously published sequences of other mammalian species especially to that of other ruminants. Localization of leptin and its receptor mRNA transcripts, as determined by in situ hybridization procedure, revealed that leptin and its receptor transcripts are expressed specifically in the alveolar epithelial cells of the mammary gland. These morphological data support that leptin could also act as an autocrine and paracrine mediator for mammary gland metabolism and as a facilitator of alveolar epithelial cell activity during lactation.

AB - Originally an overall metabolic control was attributed to the leptin hormone, which is produced mainly by the adipose tissue. Recently, leptin gene expression was demonstrated in several additional peripheral tissues. Furthermore, several isoforms of leptin receptor were found both in the central nervous system and in the peripheral tissues. Using reverse transcription and polymerase chain reaction analysis we demonstrate that leptin is expressed both in the adipose tissue and in the lactating mammary gland tissue of Egyptian water buffalo. Our results show that, short and long isoforms of leptin receptor are expressed in buffalo mammary gland tissue. We have partially cloned the buffalo leptin and its short and long isoforms of receptor, which show a high sequence homology to previously published sequences of other mammalian species especially to that of other ruminants. Localization of leptin and its receptor mRNA transcripts, as determined by in situ hybridization procedure, revealed that leptin and its receptor transcripts are expressed specifically in the alveolar epithelial cells of the mammary gland. These morphological data support that leptin could also act as an autocrine and paracrine mediator for mammary gland metabolism and as a facilitator of alveolar epithelial cell activity during lactation.

KW - Buffalo

KW - Lactation

KW - Leptin

KW - Leptin receptor

KW - Mammary gland

UR - http://www.scopus.com/inward/record.url?scp=0142058920&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0142058920&partnerID=8YFLogxK

U2 - 10.1016/S0739-7240(03)00057-2

DO - 10.1016/S0739-7240(03)00057-2

M3 - Article

C2 - 14550514

AN - SCOPUS:0142058920

VL - 25

SP - 303

EP - 314

JO - Domestic Animal Endocrinology

JF - Domestic Animal Endocrinology

SN - 0739-7240

IS - 3

ER -