Partial characterization of circulating macromolecular β2-microglobulin complexes by density gradient ultracentrifugation and page-blotting

A. Falus, J. M. Rasmussen, S. E. Svehag

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

β2-microglobulin (β2m)-containing, rapidly sedimenting peaks were seen when sera of patients with 3% polyethylene glycol (PEG)-insoluble β2m were fractionated by sucrose density gradient ultracentrifugation. No such material was found in serum of a healthy volunteer without 3% PEG-insoluble β2m. Mw of β2m-containing high molecular weight fractions ranged from 1.6 × 105 to 2.1 × 106. When some fractions were recentrifuged a majority of β2m was recovered at the original sedimentation position. Analysis of high molecular weight β2m-containing peaks by SDS-PAGE followed by electroblotting revealed only monomeric β2m indicating that β2m was not covalently bound in the high molecular weight material.

Original languageEnglish
Pages (from-to)61-64
Number of pages4
JournalImmunology letters
Volume7
Issue number2
DOIs
Publication statusPublished - 1983

Keywords

  • complexes
  • electroblot
  • ultracentrifuge
  • β2-microglobulin

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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