β2-microglobulin (β2m)-containing, rapidly sedimenting peaks were seen when sera of patients with 3% polyethylene glycol (PEG)-insoluble β2m were fractionated by sucrose density gradient ultracentrifugation. No such material was found in serum of a healthy volunteer without 3% PEG-insoluble β2m. Mw of β2m-containing high molecular weight fractions ranged from 1.6 × 105 to 2.1 × 106. When some fractions were recentrifuged a majority of β2m was recovered at the original sedimentation position. Analysis of high molecular weight β2m-containing peaks by SDS-PAGE followed by electroblotting revealed only monomeric β2m indicating that β2m was not covalently bound in the high molecular weight material.
ASJC Scopus subject areas
- Immunology and Allergy