Parallel expression of αIIβ3 and αvβ3 integrins in human melanoma cells upregulates bFGF expression and promotes their angiogenic phenotype

Balázs Döme, Erzsébet Rásó, Judit Dobos, Livia Mészáros, Norbert Varga, László G. Puskás, László Z. Fehér, Tamár Lörincz, Andrea Ladányi, Mohit Trikha, Kenneth V. Honn, József T́imár

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Previous studies indicated that transfection of the platelet integrin αIIbβ3 into human melanoma cells expressing integrin αvβ3 promoted their In vivo (but not in vitro) growth and cell survival. To reveal the underlying pathomechanism, we have analyzed the angiogenic phenotype of αIIbβ3 integrin-transduced human melanoma cells expressing integrin αvβ3. Upon heterotopic or orthotopic (intracutaneous) injections into SCID mice, the αIIbβ3 integrin-overexpressing clones, ESL, ESH, 19L and 19H, grew more rapidly than the mock transfectant (αvβ3 expressing) clone, 3.1P. Morphometry demonstrated an increased intratumoral microvessel density in 19L and 19H tumors compared to 3.1P. Immunocytochemistry and flow cytometry indicated that vascular endothelial growth factor (VEGF) is constitutively expressed in the majority of the cells of both the mock and the αIIbβ3 integrin-transfected clones. However, the mock transfectant clone, 3.1P, did not express basic flbroblast growth factor (bFGF) at protein level (

Original languageEnglish
Pages (from-to)27-35
Number of pages9
JournalInternational Journal of Cancer
Volume116
Issue number1
DOIs
Publication statusPublished - Aug 10 2005

Fingerprint

Integrins
Melanoma
Intercellular Signaling Peptides and Proteins
Up-Regulation
Phenotype
Clone Cells
SCID Mice
Microvessels
Vascular Endothelial Growth Factor A
Transfection
Cell Survival
Flow Cytometry
Blood Platelets
Immunohistochemistry
Injections
Growth
Neoplasms
Proteins

Keywords

  • β3 integrins
  • bFGF
  • Human melanoma
  • Vascularization
  • Xenograft

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Parallel expression of αIIβ3 and αvβ3 integrins in human melanoma cells upregulates bFGF expression and promotes their angiogenic phenotype. / Döme, Balázs; Rásó, Erzsébet; Dobos, Judit; Mészáros, Livia; Varga, Norbert; Puskás, László G.; Fehér, László Z.; Lörincz, Tamár; Ladányi, Andrea; Trikha, Mohit; Honn, Kenneth V.; T́imár, József.

In: International Journal of Cancer, Vol. 116, No. 1, 10.08.2005, p. 27-35.

Research output: Contribution to journalArticle

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