Paradoxical increase of heat-shock response with age in a substrain of F344 rats: Comparison between F344/DuCrj and F344/Jcl

Ryoya Takahashi, Emi Toyoda, Yasunobu Aoki, Kazuo T. Suzuki, S. Goto

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

The ability of hepatocytes isolated from young (7-10 months) and old (31 months) male F344/Jcl and F344/DuCrj rats to express heat shock protein (hsp) 27, hsp70 and hsp90 was determined after a mild heat shock (42.5°C for 30 min). The induction of these three mRNA levels by the heat shock was 50-80% lower in hepatocytes isolated from old F344/Jcl rats than in those from young rats. However, the hepatocytes from old F344/DuCrj showed a marked increase (200-250%) in the induction of hsp mRNAs by heat shock when compared to cells from young rats. Because heat shock transcription factor (HSF) plays a critical role in regulating the transcription of hsp genes, the effect of age on the binding activity HSF to heat shock element (HSE) was also studied. Again, the induction of binding activity of HSF to HSE was significantly increased with age in hepatocytes from F344/DuCrj rats while the reverse was true for the cells from F344/Jcl. The induced levels of hsp mRNAs were positively correlated with the binding activity of HSF to HSE in hepatocyte extracts from both F344 substrains, suggesting that the diverse age-related changes of heat-shock response in F344 substrains occurs in HSF activity. The contradictory age-related change in the heat-shock response is discussed with the differences in biochemical and genetic properties of substrains of F344 rats.

Original languageEnglish
Pages (from-to)1605-1615
Number of pages11
JournalMechanisms of Ageing and Development
Volume123
Issue number12
DOIs
Publication statusPublished - Nov 2002

Fingerprint

Heat-Shock Response
Inbred F344 Rats
Rats
Shock
Hot Temperature
Hepatocytes
Heat-Shock Proteins
Messenger RNA
HSP27 Heat-Shock Proteins
Molecular Biology
Transcription
heat shock transcription factor
Genes

Keywords

  • F344 rat
  • Heat shock factor
  • Heat shock protein
  • Hepatocyte
  • Liver
  • Substrain difference

ASJC Scopus subject areas

  • Ageing
  • Biochemistry
  • Developmental Biology
  • Developmental Neuroscience

Cite this

Paradoxical increase of heat-shock response with age in a substrain of F344 rats : Comparison between F344/DuCrj and F344/Jcl. / Takahashi, Ryoya; Toyoda, Emi; Aoki, Yasunobu; Suzuki, Kazuo T.; Goto, S.

In: Mechanisms of Ageing and Development, Vol. 123, No. 12, 11.2002, p. 1605-1615.

Research output: Contribution to journalArticle

Takahashi, Ryoya ; Toyoda, Emi ; Aoki, Yasunobu ; Suzuki, Kazuo T. ; Goto, S. / Paradoxical increase of heat-shock response with age in a substrain of F344 rats : Comparison between F344/DuCrj and F344/Jcl. In: Mechanisms of Ageing and Development. 2002 ; Vol. 123, No. 12. pp. 1605-1615.
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