Paracrine transactivation of the CB1 cannabinoid receptor by AT1 angiotensin and other Gq/11 protein-coupled receptors

Gábor Turu, P. Várnai, Pál Gyombolai, László Szidonya, Lásló Offertaler, G. Bagdy, George Kunos, L. Hunyady

Research output: Contribution to journalArticle

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Abstract

Intracellular signaling systems of G protein-coupled receptors are well established, but their role in paracrine regulation of adjacent cells is generally considered as a tissue-specific mechanism. We have shown previously that AT1 receptor (AT1R) stimulation leads to diacylglycerol lipase-mediated transactivation of co-expressed CB1Rs in Chinese hamster ovary cells. In the present study we detected a paracrine effect of the endocannabinoid release from Chinese hamster ovary, COS7, and HEK293 cells during the stimulation of AT1 angiotensin receptors by determining CB1 cannabinoid receptor activity with bioluminescence resonance energy transfer-based sensors of G protein activation expressed in separate cells. The angiotensin II-induced, paracrine activation of CB1 receptors was visualized by detecting translocation of green fluorescent protein-tagged β-arrestin2. Mass spectrometry analyses have demonstrated angiotensin II-induced stimulation of 2-arachidonoylglycerol production, whereas no increase of anandamide levels was observed. Stimulation of Gq/11-coupledM1, M3, M5 muscarinic, V1 vasopressin, α1a adrenergic, B2 bradykinin receptors, but not Gi/o-coupledM2 andM4 muscarinic receptors, also led to paracrine transactivation of CB1 receptors. These data suggest that, in addition to their retrograde neurotransmitter role, endocannabinoids have much broader paracrine mediator functions during activation of Gq/11-coupled receptors.

Original languageEnglish
Pages (from-to)16914-16921
Number of pages8
JournalJournal of Biological Chemistry
Volume284
Issue number25
DOIs
Publication statusPublished - Jun 19 2009

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Gq-G11 GTP-Binding Protein alpha Subunits
Cannabinoid Receptor CB1
Angiotensins
Transcriptional Activation
Endocannabinoids
Chemical activation
Cricetulus
Angiotensin II
Ovary
Cells
Bradykinin B2 Receptors
Bioluminescence
Angiotensin Type 1 Receptor
Proteins
Lipoprotein Lipase
HEK293 Cells
Energy Transfer
Muscarinic Receptors
G-Protein-Coupled Receptors
Green Fluorescent Proteins

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Molecular Biology
  • Medicine(all)

Cite this

Paracrine transactivation of the CB1 cannabinoid receptor by AT1 angiotensin and other Gq/11 protein-coupled receptors. / Turu, Gábor; Várnai, P.; Gyombolai, Pál; Szidonya, László; Offertaler, Lásló; Bagdy, G.; Kunos, George; Hunyady, L.

In: Journal of Biological Chemistry, Vol. 284, No. 25, 19.06.2009, p. 16914-16921.

Research output: Contribution to journalArticle

Turu, Gábor ; Várnai, P. ; Gyombolai, Pál ; Szidonya, László ; Offertaler, Lásló ; Bagdy, G. ; Kunos, George ; Hunyady, L. / Paracrine transactivation of the CB1 cannabinoid receptor by AT1 angiotensin and other Gq/11 protein-coupled receptors. In: Journal of Biological Chemistry. 2009 ; Vol. 284, No. 25. pp. 16914-16921.
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