LA LITHOSTATHINE PANCREATIQUE INHIBITEUR DE LA PRECIPITATION DU CACO3: RELATIONS STRUCTURE-FONCTION

Translated title of the contribution: Pancreatic lithostathine an inhibitor of CaCO3 crystal formation; structure-function relationship

J. P. Bernard, T. Takacs, M. De Reggi, H. Sarles, J. C. Dagorn

Research output: Contribution to journalArticle

Abstract

Pancreatic juice is naturally supersatured in calcium and bicarbonate ions. A mechanism controlling CaCO3 crystal formation and growth is therefore necessary to prevent duct clogging. Lithostathine, a glycoprotein synthesized by acinar cells and secreted in pancreatic juice, could be involved in such a control. Lithostathine significantly delayed crystal nucleation and inhibited growth of CaCO3 crystals from supersatured solutions. Lithostathine adsorbed to sites specifically inhibiting crystal growth with a dissociation constant Kd = 0.9 x 10-6 mol/L. The glycosylated N-terminal undecapeptide generated by limited trypsin hydrolysis of lithostathine, inhibited CaCO3 crystal growth with a Kd = 3.4 x 10-6 mol/L similar to that of lithostathine. On the contrary, the carboxy-terminal polypeptide (lithostathine H) was inactive. The N-terminal undecapeptide of lithostathine is therefore essential to the inhibitory activity of the protein on CaCO3 crystal growth.

Original languageFrench
Pages (from-to)257-259
Number of pages3
JournalNephrologie
Volume14
Issue number6
Publication statusPublished - Dec 1 1993

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Keywords

  • crystal growth
  • lithostathine
  • pancreas

ASJC Scopus subject areas

  • Nephrology

Cite this

Bernard, J. P., Takacs, T., De Reggi, M., Sarles, H., & Dagorn, J. C. (1993). LA LITHOSTATHINE PANCREATIQUE INHIBITEUR DE LA PRECIPITATION DU CACO3: RELATIONS STRUCTURE-FONCTION. Nephrologie, 14(6), 257-259.