LA LITHOSTATHINE PANCREATIQUE INHIBITEUR DE LA PRECIPITATION DU CACO3

RELATIONS STRUCTURE-FONCTION

Translated title of the contribution: Pancreatic lithostathine an inhibitor of CaCO3 crystal formation; structure-function relationship

J. P. Bernard, T. Takács, M. De Reggi, H. Sarles, J. C. Dagorn

Research output: Contribution to journalArticle

Abstract

Pancreatic juice is naturally supersatured in calcium and bicarbonate ions. A mechanism controlling CaCO3 crystal formation and growth is therefore necessary to prevent duct clogging. Lithostathine, a glycoprotein synthesized by acinar cells and secreted in pancreatic juice, could be involved in such a control. Lithostathine significantly delayed crystal nucleation and inhibited growth of CaCO3 crystals from supersatured solutions. Lithostathine adsorbed to sites specifically inhibiting crystal growth with a dissociation constant Kd = 0.9 x 10-6 mol/L. The glycosylated N-terminal undecapeptide generated by limited trypsin hydrolysis of lithostathine, inhibited CaCO3 crystal growth with a Kd = 3.4 x 10-6 mol/L similar to that of lithostathine. On the contrary, the carboxy-terminal polypeptide (lithostathine H) was inactive. The N-terminal undecapeptide of lithostathine is therefore essential to the inhibitory activity of the protein on CaCO3 crystal growth.

Original languageFrench
Pages (from-to)257-259
Number of pages3
JournalNephrologie
Volume14
Issue number6
Publication statusPublished - 1993

Fingerprint

Lithostathine
Crystallization
Pancreatic Juice
Acinar Cells
Bicarbonates
Trypsin
Glycoproteins
Hydrolysis
Peptides

Keywords

  • crystal growth
  • lithostathine
  • pancreas

ASJC Scopus subject areas

  • Nephrology

Cite this

Bernard, J. P., Takács, T., De Reggi, M., Sarles, H., & Dagorn, J. C. (1993). LA LITHOSTATHINE PANCREATIQUE INHIBITEUR DE LA PRECIPITATION DU CACO3: RELATIONS STRUCTURE-FONCTION. Nephrologie, 14(6), 257-259.

LA LITHOSTATHINE PANCREATIQUE INHIBITEUR DE LA PRECIPITATION DU CACO3 : RELATIONS STRUCTURE-FONCTION. / Bernard, J. P.; Takács, T.; De Reggi, M.; Sarles, H.; Dagorn, J. C.

In: Nephrologie, Vol. 14, No. 6, 1993, p. 257-259.

Research output: Contribution to journalArticle

Bernard, JP, Takács, T, De Reggi, M, Sarles, H & Dagorn, JC 1993, 'LA LITHOSTATHINE PANCREATIQUE INHIBITEUR DE LA PRECIPITATION DU CACO3: RELATIONS STRUCTURE-FONCTION', Nephrologie, vol. 14, no. 6, pp. 257-259.
Bernard, J. P. ; Takács, T. ; De Reggi, M. ; Sarles, H. ; Dagorn, J. C. / LA LITHOSTATHINE PANCREATIQUE INHIBITEUR DE LA PRECIPITATION DU CACO3 : RELATIONS STRUCTURE-FONCTION. In: Nephrologie. 1993 ; Vol. 14, No. 6. pp. 257-259.
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