Palmitoylation targets CD39/endothelial ATP diphosphohydrolase to caveolae

Katarzyna Koziak, Elzbieta Kaczmarek, A. Kittel, Jean Sévigny, Jan Krzysztof Blusztajn, Jan Schulte am Esch, Masato Imai, Olaf Guckelberger, Christian Goepfert, Imrana Qawi, Simon C. Robson

Research output: Contribution to journalArticle

76 Citations (Scopus)

Abstract

Ectonucleotidases influence purinergic receptor function by the hydrolysis of extracellular nucleotides. CD39 is an integral membrane protein that is a prototype member of the nucleoside 5'-triphosphate diphosphohydrolase family. The native CD39 protein has two intracytoplasmic and two transmembrane domains. There is a large extracellular domain that undergoes extensive glycosylation and can be post-translationally modified by limited proteolysis. We have identified a potential thioester linkage site for S-acylation within the N-terminal region of CD39 and demonstrate that this region undergoes palmitoylation in a constitutive manner. The covalent lipid modification of this region of the protein appears to be important both in plasma membrane association and in targeting CD39 to caveolae. These specialized plasmalemmal domains are enriched in G protein-coupled receptors and appear to integrate cellular activation events. We suggest that palmitoylation could modulate the function of CD39 in regulating cellular signal transduction pathways.

Original languageEnglish
Pages (from-to)2057-2062
Number of pages6
JournalJournal of Biological Chemistry
Volume275
Issue number3
DOIs
Publication statusPublished - Jan 21 2000

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Lipoylation
Apyrase
Caveolae
Proteolysis
Glycosylation
Purinergic Receptors
Acylation
Signal transduction
Cell membranes
G-Protein-Coupled Receptors
Nucleosides
Hydrolysis
Signal Transduction
Membrane Proteins
Proteins
Nucleotides
Chemical activation
Cell Membrane
Association reactions
Lipids

ASJC Scopus subject areas

  • Biochemistry

Cite this

Koziak, K., Kaczmarek, E., Kittel, A., Sévigny, J., Blusztajn, J. K., Schulte am Esch, J., ... Robson, S. C. (2000). Palmitoylation targets CD39/endothelial ATP diphosphohydrolase to caveolae. Journal of Biological Chemistry, 275(3), 2057-2062. https://doi.org/10.1074/jbc.275.3.2057

Palmitoylation targets CD39/endothelial ATP diphosphohydrolase to caveolae. / Koziak, Katarzyna; Kaczmarek, Elzbieta; Kittel, A.; Sévigny, Jean; Blusztajn, Jan Krzysztof; Schulte am Esch, Jan; Imai, Masato; Guckelberger, Olaf; Goepfert, Christian; Qawi, Imrana; Robson, Simon C.

In: Journal of Biological Chemistry, Vol. 275, No. 3, 21.01.2000, p. 2057-2062.

Research output: Contribution to journalArticle

Koziak, K, Kaczmarek, E, Kittel, A, Sévigny, J, Blusztajn, JK, Schulte am Esch, J, Imai, M, Guckelberger, O, Goepfert, C, Qawi, I & Robson, SC 2000, 'Palmitoylation targets CD39/endothelial ATP diphosphohydrolase to caveolae', Journal of Biological Chemistry, vol. 275, no. 3, pp. 2057-2062. https://doi.org/10.1074/jbc.275.3.2057
Koziak K, Kaczmarek E, Kittel A, Sévigny J, Blusztajn JK, Schulte am Esch J et al. Palmitoylation targets CD39/endothelial ATP diphosphohydrolase to caveolae. Journal of Biological Chemistry. 2000 Jan 21;275(3):2057-2062. https://doi.org/10.1074/jbc.275.3.2057
Koziak, Katarzyna ; Kaczmarek, Elzbieta ; Kittel, A. ; Sévigny, Jean ; Blusztajn, Jan Krzysztof ; Schulte am Esch, Jan ; Imai, Masato ; Guckelberger, Olaf ; Goepfert, Christian ; Qawi, Imrana ; Robson, Simon C. / Palmitoylation targets CD39/endothelial ATP diphosphohydrolase to caveolae. In: Journal of Biological Chemistry. 2000 ; Vol. 275, No. 3. pp. 2057-2062.
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