PACAP-mediated neuroprotection of neurochemically identified cell types in MSG-induced retinal degeneration

Tamas Atlasz, K. Szabadfi, P. Kiss, Norbert Babai, Zsombor Koszegi, A. Tamás, D. Reglodi, R. Gábriel

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

Pituitary adenylate cyclase-activating polypeptide (PACAP) is neuroprotective in animal models of different brain pathologies and injuries, including cerebral ischemia, Parkinson's disease, and different types of retinal degenerations. We have previously shown that PACAP is protective against monosodium glutamate (MSG)-induced retinal degeneration, where PACAP-treated retinas has more retained structure and PACAP induces anti-apoptotic while it inhibits pro-apoptotic signaling pathways. The aim of the present study was to investigate cell-type specific effects of PACAP in MSG-induced retinal degeneration by means of immunohistochemistry. Rat pups received MSG (2 mg/g b.w.) applied on postnatal days 1, 5, and 9. PACAP (100 pmol in 5 μl saline) was injected into the right vitreous body, while the left eye received only saline. Retinas were processed for immunocytochemistry after 3 weeks. Immunolabeling was determined for vesicular glutamate transporter 1, tyrosine hydroxylase, calretinin, calbindin, parvalbumin, and vesicular γ-aminobutyric acid (GABA) transporter. In the MSG-treated retinas, the cell bodies and processes in the inner nuclear, inner plexiform, and ganglion cell layers displayed less immunoreactivity for all antisera. Apart from photoreceptors, only one major retinal cell type examined in this study; the calbindin-immunoreactive horizontal cell seemed not to be affected by MSG application. After simultaneous application of MSG and PACAP, staining of retinas was similar to that of normal eyes, with no significant alterations in immunoreactive patterns. These findings further support the neuroprotective function of PACAP in MSG-induced retinal degeneration.

Original languageEnglish
Pages (from-to)97-104
Number of pages8
JournalJournal of Molecular Neuroscience
Volume36
Issue number1-3
DOIs
Publication statusPublished - Nov 2008

Fingerprint

Pituitary Adenylate Cyclase-Activating Polypeptide
Sodium Glutamate
Retinal Degeneration
Retina
Calbindins
Vesicular Glutamate Transport Protein 1
Immunohistochemistry
GABA Plasma Membrane Transport Proteins
Aminobutyrates
Calbindin 2
Parvalbumins
Vitreous Body
Neuroprotection
Tyrosine 3-Monooxygenase
Brain Ischemia
Ganglia
Brain Injuries
Parkinson Disease
Immune Sera
Animal Models

Keywords

  • Excitotoxicity
  • MSG
  • Neuropeptide
  • PACAP
  • Retinoprotection

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience

Cite this

PACAP-mediated neuroprotection of neurochemically identified cell types in MSG-induced retinal degeneration. / Atlasz, Tamas; Szabadfi, K.; Kiss, P.; Babai, Norbert; Koszegi, Zsombor; Tamás, A.; Reglodi, D.; Gábriel, R.

In: Journal of Molecular Neuroscience, Vol. 36, No. 1-3, 11.2008, p. 97-104.

Research output: Contribution to journalArticle

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