PACAP Is Protective in a Rat Model of Retinopathy of Prematurity

Timea Kvarik, Barbara Mammel, D. Reglodi, Krisztina Kovacs, Dora Werling, Brigitta Bede, Alexandra Vaczy, Eszter Fabian, G. Tóth, P. Kiss, A. Tamás, Tibor Ertl, Judit Gyarmati, Tamas Atlasz

Research output: Contribution to journalArticle

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Abstract

The oxygen-induced retinopathy (OIR) is a well-established rodent model of retinopathy of prematurity (ROP), which is one of the most common causes of childhood visual impairment affecting preterm babies. Pituitary adenylate cyclase-activating polypeptide (PACAP) is known to have neuroprotective effects. Several studies have revealed the presence of PACAP and its receptors in the retina and reported its protective effects in ischemic and diabetic retinopathy. In this study, we investigated whether PACAP administration can influence the vascular changes in the rat OIR model. OIR was generated by placing the animals in daily alternating 10/50 oxygen concentrations from postnatal day (PD) 0 to PD14 then returned them to room air. Meanwhile, animals received PACAP or saline intraperitoneally or intravitreally from PD1 to PD8 or on PD11, PD14, and PD17, respectively. On PD19 ± 1, the retinas were isolated and the vessels were visualized by isolectin staining. The percentage of avascular to whole retinal areas and the number of branching points were measured. Change in cytokine expression was also determined. Intravitreal treatment with PACAP remarkably reduced the extent of avascular area compared to the non- and saline-treated OIR groups. Intraperitoneal PACAP injection did not influence the vascular extent. Retinal images of room-air controls did not show vascular alterations. No changes in the number of vessel branching were observed after treatments. Alterations in cytokine profile after local PACAP injection further supported the protective role of the peptide. This is the first study to examine the effects of PACAP in ROP. Although the exact mechanism is still not revealed, the present results show that PACAP treatment can ameliorate the vascular changes in the animal model of ROP.

Original languageEnglish
Pages (from-to)179-185
Number of pages7
JournalJournal of Molecular Neuroscience
Volume60
Issue number2
DOIs
Publication statusPublished - Oct 1 2016

Fingerprint

Pituitary Adenylate Cyclase-Activating Polypeptide
Retinopathy of Prematurity
Blood Vessels
Oxygen
Retina
Pituitary Adenylate Cyclase-Activating Polypeptide Receptors
Air
Cytokines
Injections
Vision Disorders
Neuroprotective Agents
Diabetic Retinopathy
Lectins
Rodentia
Therapeutics
Animal Models
Staining and Labeling
Peptides

Keywords

  • Muller glial cell
  • Neovascularization
  • Oxygen-induced retinopathy
  • Retina

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience

Cite this

Kvarik, T., Mammel, B., Reglodi, D., Kovacs, K., Werling, D., Bede, B., ... Atlasz, T. (2016). PACAP Is Protective in a Rat Model of Retinopathy of Prematurity. Journal of Molecular Neuroscience, 60(2), 179-185. https://doi.org/10.1007/s12031-016-0797-5

PACAP Is Protective in a Rat Model of Retinopathy of Prematurity. / Kvarik, Timea; Mammel, Barbara; Reglodi, D.; Kovacs, Krisztina; Werling, Dora; Bede, Brigitta; Vaczy, Alexandra; Fabian, Eszter; Tóth, G.; Kiss, P.; Tamás, A.; Ertl, Tibor; Gyarmati, Judit; Atlasz, Tamas.

In: Journal of Molecular Neuroscience, Vol. 60, No. 2, 01.10.2016, p. 179-185.

Research output: Contribution to journalArticle

Kvarik, T, Mammel, B, Reglodi, D, Kovacs, K, Werling, D, Bede, B, Vaczy, A, Fabian, E, Tóth, G, Kiss, P, Tamás, A, Ertl, T, Gyarmati, J & Atlasz, T 2016, 'PACAP Is Protective in a Rat Model of Retinopathy of Prematurity', Journal of Molecular Neuroscience, vol. 60, no. 2, pp. 179-185. https://doi.org/10.1007/s12031-016-0797-5
Kvarik, Timea ; Mammel, Barbara ; Reglodi, D. ; Kovacs, Krisztina ; Werling, Dora ; Bede, Brigitta ; Vaczy, Alexandra ; Fabian, Eszter ; Tóth, G. ; Kiss, P. ; Tamás, A. ; Ertl, Tibor ; Gyarmati, Judit ; Atlasz, Tamas. / PACAP Is Protective in a Rat Model of Retinopathy of Prematurity. In: Journal of Molecular Neuroscience. 2016 ; Vol. 60, No. 2. pp. 179-185.
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