PACAP improves functional outcome in excitotoxic retinal lesion: An electroretinographic study

Balazs Varga, K. Szabadfi, P. Kiss, Eszter Fabian, A. Tamás, Monika Griecs, R. Gábriel, D. Reglodi, Adam Kemeny-Beke, Zsuzsanna Pamer, Zsolt Biro, A. Tósaki, Tamas Atlasz, B. Juhász

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Pituitary adenylate cyclase-activating polypeptide (PACAP) and its receptors occur throughout the nervous system, including the retina. PACAP exerts diverse actions in the eye: it influences ocular blood flow, contraction of the ciliary muscle, and has retinoprotective effects. This has been proven in different models of retinal degeneration. The in vivo protective effects of PACAP have been shown in retinal degeneration induced by kainic acid, optic nerve transection and ischemia. We have previously shown by morphological, morphometrical and immunohistochemical analyses that intravitreal PACAP administration protects against monosodium glutamate (MSG)-induced damage in neonatal rats. The question was raised whether these apparent morphological improvements by PACAP administration also lead to functional amelioration in MSG-induced retinal damage. The aim of the present study was to investigate the functional consequences of MSG treatment and the subsequent PACAP administration using electroretinographic measurements. The histological and morphometrical analyses supported the earlier findings that PACAP protected the retina in MSG-induced excitotoxicity. ERG recordings revealed a marked decrease in both the b- and a-wave values, reflecting the function of the inner retinal layers and the photoreceptors, respectively. In retinas receiving intravitreal PACAP treatment, these values were significantly increased. Thus, the functional outcome, although not parallel with the morphology, was significantly improved after PACAP treatment. The present observations are important from the clinical point of view showing, for the first time, that PACAP treatment is able to improve the functional properties of the retina in excitotoxic damage.

Original languageEnglish
Pages (from-to)44-50
Number of pages7
JournalJournal of Molecular Neuroscience
Volume43
Issue number1
DOIs
Publication statusPublished - Jan 2011

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Pituitary Adenylate Cyclase-Activating Polypeptide
Sodium Glutamate
Retina
Retinal Degeneration
Pituitary Adenylate Cyclase-Activating Polypeptide Receptors
Optic Nerve Injuries
Ischemic Optic Neuropathy
Vertebrate Photoreceptor Cells
Kainic Acid
Muscle Contraction
Nervous System

Keywords

  • Electroretinography
  • Excitotoxicity
  • Neurotrophic factor
  • Retinoprotection

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience

Cite this

PACAP improves functional outcome in excitotoxic retinal lesion : An electroretinographic study. / Varga, Balazs; Szabadfi, K.; Kiss, P.; Fabian, Eszter; Tamás, A.; Griecs, Monika; Gábriel, R.; Reglodi, D.; Kemeny-Beke, Adam; Pamer, Zsuzsanna; Biro, Zsolt; Tósaki, A.; Atlasz, Tamas; Juhász, B.

In: Journal of Molecular Neuroscience, Vol. 43, No. 1, 01.2011, p. 44-50.

Research output: Contribution to journalArticle

Varga, Balazs ; Szabadfi, K. ; Kiss, P. ; Fabian, Eszter ; Tamás, A. ; Griecs, Monika ; Gábriel, R. ; Reglodi, D. ; Kemeny-Beke, Adam ; Pamer, Zsuzsanna ; Biro, Zsolt ; Tósaki, A. ; Atlasz, Tamas ; Juhász, B. / PACAP improves functional outcome in excitotoxic retinal lesion : An electroretinographic study. In: Journal of Molecular Neuroscience. 2011 ; Vol. 43, No. 1. pp. 44-50.
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