PACAP-27 radioimmunoassay: Description and application of a novel method

J. Németh, B. Jakab, R. Józsa, T. Hollósy, A. Tamás, A. Lubics, I. Lengvári, P. Kiss, Zs Oberritter, B. Horváth, Z. Szilvássy, D. Reglodi

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Pituitary adenylate cyclase activating polypeptide (PACAP) is a widely distributed neuropeptide that has two molecular forms with 38 and 27 amino acid residues. The aim of the present study was to develop a new, highly specific PACAP-27 assay to investigate the quantitative distribution of PACAP-27 in the central nervous system of various vertebrate species applying the same technical and experimental conditions. Our results show that the antiserum used turned to be PACAP-27 specific. The average ID50 value was 51.5±3.6 fmol/ml and the detection limit was 2 fmol/ml. PACAP-27 immunoreactivity was present in the examined brain areas, with highest concentration in the rat diencephalon and telencephalon. Swine and pigeon brain also contained significant amount of PACAP-27. Our results confirm the previously described data showing that PACAP-38 is the dominant form of PACAP in vertebrates, since PACAP-38 levels exceeded those of PACAP-27 in all examined brain areas. Furthermore, our study describes for the first time, the comparative quantitative distribution of PACAP-27 and-38 in the swine and pigeon brain.

Original languageEnglish
Pages (from-to)327-332
Number of pages6
JournalJournal of Radioanalytical and Nuclear Chemistry
Volume273
Issue number2
DOIs
Publication statusPublished - Aug 1 2007

ASJC Scopus subject areas

  • Analytical Chemistry
  • Nuclear Energy and Engineering
  • Radiology Nuclear Medicine and imaging
  • Pollution
  • Spectroscopy
  • Public Health, Environmental and Occupational Health
  • Health, Toxicology and Mutagenesis

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