PAC1 receptor localization in a model nervous system

Light and electron microscopic immunocytochemistry on the earthworm ventral nerve cord ganglia

L. Molnár, E. Pollák, A. Boros, S. Shioda, S. Nakajo, A. Tamás, I. Lengvári, D. Reglodi, A. Lubics

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

The presence and pattern of pituitary adenylate cyclase activating polypeptide (PACAP) type I (PAC1) receptors were identified by means of pre- and post-embedding immunocytochemical methods in the ventral nerve cord ganglia (VNC) of the earthworm Eisenia fetida. Light and electron microscopic observations revealed the exact anatomical positions of labeled structures suggesting that PACAP mediates the activity of some interneurons, a few small motoneurons and certain sensory fibers that are located in ventrolateral, ventromedial and intermediomedial sensory longitudinal axon bundles of the VNC ganglia. No labeling was located on large interneuronal systems such as dorsal medial and lateral giant axon systems and ventral giant axons. At the ultrastructural level labeling was mainly restricted to endo- and plasma membranes showing characteristic unequal distribution in various neuron parts. An increasing abundance of PAC1 receptors located on both rough endoplasmic reticulum and plasma membranes was seen from perikarya to neural processes, indicating that intracellular membrane traffic might play a crucial role in the transportation of PAC1 receptors. High number of PAC1 receptors was found in both pre- and postsynaptic membranes in addition to extrasynaptic sites suggesting that PACAP acts as neurotransmitter and neuromodulator in the earthworm nervous system.

Original languageEnglish
Pages (from-to)96-104
Number of pages9
JournalRegulatory Peptides
Volume145
Issue number1-3
DOIs
Publication statusPublished - Jan 10 2008

Fingerprint

Receptors, Pituitary Adenylate Cyclase-Activating Polypeptide, Type I
Oligochaeta
Neurology
Pituitary Adenylate Cyclase-Activating Polypeptide
Ganglia
Nervous System
Immunohistochemistry
Electrons
Axons
Light
Cell membranes
Labeling
Neurotransmitter Agents
Cell Membrane
Membranes
Intracellular Membranes
Rough Endoplasmic Reticulum
Motor Neurons
Interneurons
Neurons

Keywords

  • Electron microscopy
  • Immunogold labeling
  • Model nervous system
  • Pre- and post-embedding immunocytochemistry

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology
  • Physiology
  • Neuroscience(all)

Cite this

PAC1 receptor localization in a model nervous system : Light and electron microscopic immunocytochemistry on the earthworm ventral nerve cord ganglia. / Molnár, L.; Pollák, E.; Boros, A.; Shioda, S.; Nakajo, S.; Tamás, A.; Lengvári, I.; Reglodi, D.; Lubics, A.

In: Regulatory Peptides, Vol. 145, No. 1-3, 10.01.2008, p. 96-104.

Research output: Contribution to journalArticle

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