P53 protein and its messenger ribonucleic acid in human adrenal tumors

V. Adleff, K. Rácz, M. Tóth, I. Varga, A. Bezzegh, E. Gláz

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Abstract

The role of p53 tumor suppressor gene in the pathomechanism of adrenal tumors was investigated by measuring p53 protein and its messenger ribonucleic acid (mRNA) in 12 normal human adrenals as well as in 56 adrenal tumors (7 aldosterone-producing adenomas, 5 adrenocortical adenomas causing Gushing's syndrome, 19 non-hyperfunctioning adrenocortical adenomas, 5 adrenocortical carcinomas, 12 pheochromocytomas, 3 myelolipomas, 4 ganglioneuromas and 1 hemangioma). The p53 protein concentration was significantly increased in aldosterone-producing adenomas (394 ± 36 pg/mg cytosolic protein, mean ± SE, vs 266 ± 18 in normal human adrenals), whereas the concentration of this protein in Gushing's adenomas, non-hyperfunctioning adrenocortical adenomas, pheochromocytomas, and in all but one adrenocortical carcinomas was similar to that measured in normal human adrenal tissues. One adrenocortical carcinoma tissue showed very high p53 protein content (3000 pg/mg cytosolic protein). By contrast, myelolipomas (23 ± 20) ganglioneuromas (43 ± 15) and a hemangioma (11 pg/mg cytosolic protein) had very low p53 protlein content. Northern blot analysis revealed the presence of p53 mRNA in each adrenal tissue examined with highest levels in aldosterone-producing and Gushing's adenomas. It is possible that the differences in p53 protein and/or mRNA contents reflect corresponding differences in the pathogenetic importance of p53 alterations in these types of adrenal tumors.

Original languageEnglish
Pages (from-to)753-757
Number of pages5
JournalJournal of endocrinological investigation
Volume21
Issue number11
DOIs
Publication statusPublished - Jan 1 1998

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Keywords

  • Adrenal
  • Adrenal pathophysiology
  • Luminometric immunoassay
  • Tumors
  • p53 expression
  • p53 protein

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Endocrinology

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