P190RhoGAP has cellular RacGAP activity regulated by a polybasic region

Magdolna Lévay, Balázs Bartos, E. Ligeti

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

p190RhoGAP is a GTPase-activating protein (GAP) known to regulate actin cytoskeleton dynamics by decreasing RhoGTP levels through activation of the intrinsic GTPase activity of Rho. Although the GAP domain of p190RhoGAP stimulates the intrinsic' GTPase activity of several Rho family members (Rho, Rac, Cdc42) under in vitro conditions, p190RhoGAP is generally regarded as a GAP for RhoA in the cell. The cellular RacGAP activity of the protein has not been proven directly. We have previously shown that the in vitro RacGAP and RhoGAP activity of p190RhoGAP was inversely regulated through a polybasic region of the protein. Here we provide evidence that p190RhoGAP shows remarkable GAP activity toward Rac also in the cell. The cellular RacGAP activity of p190RhoGAP requires an intact polybasic region adjacent to the GAP domain whereas the RhoGAP activity is inhibited by the same domain. Our data indicate that through its alternating RacGAP and RhoGAP activity, p190RhoGAP plays a more complex role in the Rac-Rho antagonism than it was realized earlier.

Original languageEnglish
Pages (from-to)1388-1394
Number of pages7
JournalCellular Signalling
Volume25
Issue number6
DOIs
Publication statusPublished - Jun 2013

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GTPase-Activating Proteins
rho GTP-Binding Proteins
GTP Phosphohydrolases
Actin Cytoskeleton
Proteins

Keywords

  • P190RhoGAP
  • Polybasic region
  • Rac
  • Rho

ASJC Scopus subject areas

  • Cell Biology

Cite this

P190RhoGAP has cellular RacGAP activity regulated by a polybasic region. / Lévay, Magdolna; Bartos, Balázs; Ligeti, E.

In: Cellular Signalling, Vol. 25, No. 6, 06.2013, p. 1388-1394.

Research output: Contribution to journalArticle

Lévay, Magdolna ; Bartos, Balázs ; Ligeti, E. / P190RhoGAP has cellular RacGAP activity regulated by a polybasic region. In: Cellular Signalling. 2013 ; Vol. 25, No. 6. pp. 1388-1394.
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