The RHPA allows distinction of effects on the number of secreting cells (%PFC) and the amount of hormone that each cell secretes. We report the case of a 58-year-old acromegalic man. The in vitro basal GH secretion and the responsiveness of tumour cells to different releasing and inhibiting factors were analyzed; the results were compared with the pre- and post-operative in vivo data. The %PFC was significantly decreased by SRIH (15-23% vs 6-13%). PACAP is known as a stimulator of GH secretion. When tumour cells were preexposed for several h to PACAP, SRIH did not decrease, but even increased the GH release. Conclusions: (1) RHPA allows to detect the hormone secreting properties of the removed tumour tissue within a few hours. (2) In our case SRIH decreased, GHRH and PACAP increased the GH secretion. (3) Inverse SRIH effect, i.e. the increase of GH secretion was found when cells were pretreated with PACAP.
|Number of pages||1|
|Journal||Endocrinology and Metabolism, Supplement|
|Publication status||Published - Jan 1 1997|
ASJC Scopus subject areas
- Endocrinology, Diabetes and Metabolism