P-030. Analysis of growth hormone secretion of individual pituitary adenoma cells from an acromegalic patient as determined by the reverse hemolytic plaque assay (RHPA)

M. I. Goth, K. Horvath, S. Czirjak, G. Szilagyi, I. Szabolcs, L. Kovacs, O. Dohan, G. M. Nagy

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Abstract

The RHPA allows distinction of effects on the number of secreting cells (%PFC) and the amount of hormone that each cell secretes. We report the case of a 58-year-old acromegalic man. The in vitro basal GH secretion and the responsiveness of tumour cells to different releasing and inhibiting factors were analyzed; the results were compared with the pre- and post-operative in vivo data. The %PFC was significantly decreased by SRIH (15-23% vs 6-13%). PACAP is known as a stimulator of GH secretion. When tumour cells were preexposed for several h to PACAP, SRIH did not decrease, but even increased the GH release. Conclusions: (1) RHPA allows to detect the hormone secreting properties of the removed tumour tissue within a few hours. (2) In our case SRIH decreased, GHRH and PACAP increased the GH secretion. (3) Inverse SRIH effect, i.e. the increase of GH secretion was found when cells were pretreated with PACAP.

Original languageEnglish
Number of pages1
JournalEndocrinology and Metabolism, Supplement
Volume4
Issue numberA
Publication statusPublished - Jan 1 1997

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ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Endocrinology

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