Oxytocin regulates the expression of aquaporin 5 in the late-pregnant rat uterus

Eszter Ducza, Adrienn B. Seres, Judit Hajagos-Tóth, G. Falkay, R. Gáspár

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Aquaporins (AQPs) are integral membrane channels responsible for the transport of water across a cell membrane. Based on reports that AQPs are present and accumulate in the female reproductive tract late in pregnancy, our aim was to study the expression of AQP isoforms (AQP1, 2, 3, 5, 8, and 9) at the end of pregnancy in rat in order to determine if they play a role in parturition. Reverse-transcriptase PCR revealed that specific Aqp mRNAs were detectable in the myometrium of non-pregnant and late-pregnancy (Days 18, 20, 21, and 22 of pregnancy) rat uteri. The expression of Aqp5 mRNA and protein were most pronounced on Days 18-21, and were dramatically decreased on Day 22 of pregnancy. In contrast, a significant increase was found in the level of Aqp5 transcript in whole-blood samples on the last day of pregnancy. The effect of oxytocin on myometrial Aqp5 expression in an organ bath was also investigated. The level of Aqp5 mRNA significantly decreased 5min after oxytocin (10-8M) administration, similarly to its profile on the day of delivery; this effect was sensitive to the oxytocin antagonist atosiban. The vasopressin analog desmopressin (3.7×10-8M), on the other hand, did not alter the expression of Aqp5, but did increased the amount of Aqp2 mRNA, an effect that was atosiban-resistant. These results lead us to propose that oxytocin selectively influences the expression of Aqp5 at the end of pregnancy, and may participate in events that lead to parturition in the rat. The sudden increase of AQP5 in the blood on the last day of pregnancy may serve as a marker that indicates the initiation of delivery.

Original languageEnglish
Pages (from-to)524-530
Number of pages7
JournalMolecular Reproduction and Development
Volume81
Issue number6
DOIs
Publication statusPublished - 2014

Fingerprint

Aquaporin 5
Oxytocin
Uterus
Pregnancy
Aquaporins
Messenger RNA
Parturition
Deamino Arginine Vasopressin
Myometrium
Reverse Transcriptase Polymerase Chain Reaction
Vasopressins
Baths
Ion Channels
Protein Isoforms
Cell Membrane

ASJC Scopus subject areas

  • Cell Biology
  • Genetics
  • Developmental Biology
  • Medicine(all)

Cite this

Oxytocin regulates the expression of aquaporin 5 in the late-pregnant rat uterus. / Ducza, Eszter; Seres, Adrienn B.; Hajagos-Tóth, Judit; Falkay, G.; Gáspár, R.

In: Molecular Reproduction and Development, Vol. 81, No. 6, 2014, p. 524-530.

Research output: Contribution to journalArticle

Ducza, Eszter ; Seres, Adrienn B. ; Hajagos-Tóth, Judit ; Falkay, G. ; Gáspár, R. / Oxytocin regulates the expression of aquaporin 5 in the late-pregnant rat uterus. In: Molecular Reproduction and Development. 2014 ; Vol. 81, No. 6. pp. 524-530.
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