Oxygen consumption by purified plasmalemma vesicles from wheat roots. Stimulation by NADH and salicylhydroxamic acid (SHAM)

Ian M. Møller, Alajos Bérczi

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Abstract

Plasmalemma vesicles from wheat (Triticum aestivum L.) roots consumed O2 and the addition of 1 mM NADH increased the rate ~ 3-fold (to 15-30 nmol O2·mg-1·min-1). The NADH-dependent O2 uptake was abolished by catalase. In the presence of salicylhydroxamic acid (SHAM), an inhibitor of the alternative oxidase pathway in plant mitochondria, NADH-dependent O2 consumption was stimulated 10-20-fold (to 200-400 nmol·mg1̄·min-1). Catalase also abolished this stimulation, which was KCN-sensitive but antimycin A-insensitive, and the production of H2O2 during SHAM-stimulated NADH-dependent O2 uptake was demonstrated. Irrespective of the mechanism, SHAM-stimulated respiration by root plasmalemma makes it difficult to interpret results on root respiration obtained using KCN and SHAM.

Original languageEnglish
Pages (from-to)180-184
Number of pages5
JournalFEBS letters
Volume193
Issue number2
DOIs
Publication statusPublished - Dec 2 1985

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Keywords

  • (Wheat root)
  • NADH
  • Plasmalemma
  • Respiration
  • Salicylhydroxamic acid

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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