Oxidative stress induced by bloodless limb surgery on humans

E. Karg, I. Németh, G. Virag, T. Mészaros, D. Boda, S. Pintér

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Measure of oxidative stress were studied in blood samples from 10 patients undergoing bloodless lower limb surgery. Ischaemia induced a significant increase in plasma hypoxanthine concentration and xanthine oxidase activity both in the operated leg and in the systemic circulation. Five minutes after reperfusion, ratio of xanthine oxidase/total xanthine oxidase and dehydrogenase activities rose moderately, whereas at 20 min xanthine oxidase accounted for all xanthine oxidoreductase activity in the systemic circulation. A significant increase in blood glutathione redox ratio, enhanced oxidation of haemoglobin to methaemoglobin and rise in plasma haemoglobin. concentration were present only in the operated limb. Thus, although the level of the potential free radical generators rose significantly both locally and in the systemic circulation, oxidative stress, as indicated by blood glutathione and erythrocyte injuries, remained limited to the reperfused leg.

Original languageEnglish
Pages (from-to)984-991
Number of pages8
JournalEuropean Journal of Clinical Investigation
Volume27
Issue number12
DOIs
Publication statusPublished - Jan 1 1997

Keywords

  • Glutathione redox ratio
  • Ischaemia reperfusion,
  • Xanthine dehydrogenase
  • Xanthine oxidase

ASJC Scopus subject areas

  • Biochemistry
  • Clinical Biochemistry

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