Oxidative stress and antioxidant defense mechanism in glomerular diseases

S. Túri, I. Németh, Attila Torkos, Levente Sághy, I. Varga, B. Matkovics, J. Nagy

Research output: Contribution to journalArticle

74 Citations (Scopus)

Abstract

The changes in red blood cell (RBC) lipid peroxidation [measured via the malonyl dialdehyde (MDA) concentration], reduced (GSH), and oxidized glutathione (GSSG) levels, hemoglobin (Hb) oxidation and antioxidant enzyme [catalase (Cat), glutathione peroxidase, and superoxide dismutase (SOD)] activities were studied in 45 pediatric patients with various glomerular diseases [minimal change nephrotic syndrome (MCNS) in relapse or in remission, lupus nephropathy (SLE), poststreptococcal glomerulonephritis (APSGN), IgA nephropathy (IGA gn)], and in 20 adult patients with IGA gn and also in 15 pediatric and 14 adult controls. The in vitro effects of hydrogen peroxide [acetyl phenylhydrazine (APH) test] on the GSH and Hb metabolisms were likewise investigated. There was an increased oxidative stress in MCNS with relapse, IGA gn, SLE gn, and APSGN, which could be detected in the GSH and Hb oxidation and in the lipid peroxidation on the peripheral RBC-s. The RBC SOD and Cat activities were significantly lower in all patients than in the controls. The RBC GSSG level was significantly elevated in all patients, with the exception of MCNS in remission. This stimulated a compensatory GSH production in MCNS with relapse and in IGA gn, but not in SLE or APSGN. The regeneration of GSH from GSSG was reduced in MCNS with relapse, SLE, and IGA gn, but not in APSGN. In remission, the GSH-GSSG redox system normalizes, but in vitro the APH test stimulates an intensive Hb oxidation. In conclusion, there is a correlation between the presence of active glomerular disease and the evidence of oxidative changes in the various parameters measured in peripheral RBCs.

Original languageEnglish
Pages (from-to)161-168
Number of pages8
JournalFree Radical Biology and Medicine
Volume22
Issue number1-2
DOIs
Publication statusPublished - 1996

Fingerprint

Lipoid Nephrosis
Oxidative stress
Glutathione Disulfide
Oxidative Stress
Antioxidants
Hemoglobins
Blood
Erythrocytes
Recurrence
Pediatrics
Oxidation
Catalase
Lipid Peroxidation
Superoxide Dismutase
IGA Glomerulonephritis
Lipids
Glutathione Peroxidase
Glomerulonephritis
Metabolism
Immunoglobulin A

Keywords

  • Antioxidant defense
  • Free radicals
  • Glomerular disease
  • Oxidative stress

ASJC Scopus subject areas

  • Medicine(all)
  • Toxicology
  • Clinical Biochemistry

Cite this

Oxidative stress and antioxidant defense mechanism in glomerular diseases. / Túri, S.; Németh, I.; Torkos, Attila; Sághy, Levente; Varga, I.; Matkovics, B.; Nagy, J.

In: Free Radical Biology and Medicine, Vol. 22, No. 1-2, 1996, p. 161-168.

Research output: Contribution to journalArticle

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