Overview of cell synchronization

Research output: Chapter in Book/Report/Conference proceedingChapter

3 Citations (Scopus)

Abstract

The widespread interest in cell synchronization is maintained by the studies of control mechanism involved in cell cycle regulation. During the synchronization distinct subpopulations of cells are obtained representing different stages of the cell cycle. These subpopulations are then used to study regulatory mechanisms of the cycle at the level of macromolecular biosynthesis (DNA synthesis, gene expression, protein synthesis), protein phosphorylation, development of new drugs, etc. Although several synchronization methods have been described, it is of general interest that scientists get a compilation and an updated view of these synchronization techniques. This introductory chapter summarizes: (1) the basic concepts and principal criteria of cell cycle synchronizations, (2) the most frequently used synchronization methods, such as physical fractionation (flow cytometry, dielectrophoresis, cytofluorometric purification), chemical blockade, (3) synchronization of embryonic cells, (4) synchronization at low temperature, (5) comparison of cell synchrony techniques, (6) synchronization of unicellular organisms, and (7) the effect of synchronization on transfection.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages3-27
Number of pages25
Volume1524
DOIs
Publication statusPublished - 2017

Publication series

NameMethods in Molecular Biology
Volume1524
ISSN (Print)10643745

Fingerprint

Cell Cycle
Transfection
Flow Cytometry
Proteins
Phosphorylation
Gene Expression
Temperature
DNA
Pharmaceutical Preparations

Keywords

  • Basic concepts of synchronization
  • C-value
  • Criteria of synchronization
  • DNA analysis
  • DNA staining

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Bánfalvi, G. (2017). Overview of cell synchronization. In Methods in Molecular Biology (Vol. 1524, pp. 3-27). (Methods in Molecular Biology; Vol. 1524). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-6603-5_1

Overview of cell synchronization. / Bánfalvi, G.

Methods in Molecular Biology. Vol. 1524 Humana Press Inc., 2017. p. 3-27 (Methods in Molecular Biology; Vol. 1524).

Research output: Chapter in Book/Report/Conference proceedingChapter

Bánfalvi, G 2017, Overview of cell synchronization. in Methods in Molecular Biology. vol. 1524, Methods in Molecular Biology, vol. 1524, Humana Press Inc., pp. 3-27. https://doi.org/10.1007/978-1-4939-6603-5_1
Bánfalvi G. Overview of cell synchronization. In Methods in Molecular Biology. Vol. 1524. Humana Press Inc. 2017. p. 3-27. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-4939-6603-5_1
Bánfalvi, G. / Overview of cell synchronization. Methods in Molecular Biology. Vol. 1524 Humana Press Inc., 2017. pp. 3-27 (Methods in Molecular Biology).
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