Overview of cell synchronization

Research output: Chapter in Book/Report/Conference proceedingChapter

30 Citations (Scopus)

Abstract

Widespread interest in cell synchronization is maintained by the studies of control mechanisms involved in cell cycle regulation. During the synchronization distinct subpopulations of cells are obtained representing different stages of the cell cycle. These subpopulations are then used to study regulatory mechanisms of the cycle at the level of macromolecular biosynthesis (DNA synthesis, gene expression, protein synthesis), protein phosphorylation, development of new drugs, etc. Although several synchronization methods have been described, it is of general interest that scientists get a compilation and an updated view of these synchronization techniques. This introductory chapter summarizes: (1) the basic concepts and principal criteria of cell cycle synchronizations, (2) the most frequently used synchronization methods, such as physical fractionation (flow cytometry, dielectrophoresis, cytofluorometric purification), chemical blockade, (3) synchronization of embryonic cells, (4) synchronization at low temperature, (5) comparison of cell synchrony techniques, (6) synchronization of unicellular organisms, and (7) the effect of synchronization on transfection.

Original languageEnglish
Title of host publicationCell Cycle Synchronization
Subtitle of host publicationMethods and Protocols
EditorsGaspar Banfalvi
Pages1-23
Number of pages23
DOIs
Publication statusPublished - Aug 4 2011

Publication series

NameMethods in Molecular Biology
Volume761
ISSN (Print)1064-3745

Keywords

  • Basic concepts of synchronization
  • C-value
  • criteria of synchronization
  • DNA analysis
  • DNA staining

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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  • Cite this

    Banfalvi, G. (2011). Overview of cell synchronization. In G. Banfalvi (Ed.), Cell Cycle Synchronization: Methods and Protocols (pp. 1-23). (Methods in Molecular Biology; Vol. 761). https://doi.org/10.1007/978-1-61779-182-6_1