Overpressured multi-layer chromatography

Ernö Tyihák, Emil Mincsovics, Tibor J. Székely

Research output: Contribution to journalArticle

26 Citations (Scopus)


A new version of overpressured layer chromatography using two, three or more chromatoplates during one separation was developed. The admission of the eluent to the multi-layer system as a critical step is performed by making a perforation in the chromatoplates at the eluent inlet of a suitable size and shape. The technique, called overpressured multi-layer chromatography, is the most up-to-date version of layer liquid chromatography and is very attractive because a large number of samples (50-100 or more) can be separated during one development. It can be used effectively, e.g., in plant breeding, clinical laboratories and industrial control laboratories and for the sequence analysis of proteins and nucleic acids.

Original languageEnglish
Pages (from-to)375-387
Number of pages13
JournalJournal of Chromatography A
Issue numberC
Publication statusPublished - Jun 2 1989

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Organic Chemistry

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