Overpressured-layer chromatographic determination of ascorbigen (bound Vitamin C) in Brassica vegetables

György Kátay, Zsolt Németh, Szilárd Szani, Oszkár Köck, Levente Albert, Erno Tylhák

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

A simple and efficient method is described for separation and determination of ascorbigen in Brassica vegetables by overpressured-layer chromatography. Natural ascorbigen was identified from chromatographic retention, UV spectral, and mass spectrometric data using the authentic substance for comparison. Quantification of ascorbigen revealed significant differences (4.52-26.81 mg kg-1) among the species studied. The highest level of endogenous ascorbigen was observed in the sample prepared from broccoli (26.81 mg kg-1).

Original languageEnglish
Pages (from-to)360-364
Number of pages5
JournalJournal of Planar Chromatography - Modern TLC
Volume17
Issue number5
DOIs
Publication statusPublished - Sep 1 2004

Keywords

  • Ascorbigen
  • Bound vitamin C
  • Brassica vegetables
  • Overpressured-layer chromatography

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Clinical Biochemistry

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