Overpressured layer chromatographic determination of aflatoxin B1, B2, G1 and G2 in red paprika

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Abstract

Determination of aflatoxin B1 and total aflatoxin (B1 + B2 + G1 + G2) in red paprika powder is described using column chromatographic sample clean-up, overpressured layer chromatography (OPLC) separation and fluorescence densitometric evaluation. Two OPLC methods were developed for separation of the four aflatoxins. The detection limit and quantification limit of aflatoxins in red paprika were 0.5 and 1 μg/kg in both methods, respectively. Recovery experiment was carried out with sample containing 1.74 μg/kg aflatoxin B1 and 3.56 μg/kg total aflatoxins measured by European standard HPLC method. Mean recovery amounted to 78.5% (SD 16.1%, n = 5) for aflatoxin B1 and 81.8% (SD 17.1%, n = 5) for total aflatoxins in the case of method 1. It was 105.3% (SD 10.7%, n = 5) for aflatoxin B1 and 97.4% (SD 18.6%, n = 5) for total aflatoxins using the method 2. Despite of that the Hungarian climate is not proper for the toxin production of moulds high aflatoxin B1 contaminated red paprika purchased from the market was found, which may originate from mixing of imported paprika containing very high level toxin with Hungarian one.

Original languageEnglish
Pages (from-to)140-144
Number of pages5
JournalMicrochemical Journal
Volume85
Issue number1 SPEC. ISS.
DOIs
Publication statusPublished - Jan 2007

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Aflatoxin B1
Aflatoxins
Chromatography
Recovery
Powders
Fluorescence
Experiments

Keywords

  • Aflatoxin
  • Overpressured layer chromatography (OPLC)
  • Red paprika

ASJC Scopus subject areas

  • Analytical Chemistry
  • Spectroscopy

Cite this

Overpressured layer chromatographic determination of aflatoxin B1, B2, G1 and G2 in red paprika. / Móricz, A.; Fatér, Zsuzsanna; Otta, K.; Tyihák, E.; Mincsovics, E.

In: Microchemical Journal, Vol. 85, No. 1 SPEC. ISS., 01.2007, p. 140-144.

Research output: Contribution to journalArticle

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