Overlapping imprinting of oligopeptides in Chang liver cells. Data on the mechanism of hormone evolution

G. Csaba, P. Kovács, S. Tóth, S. Bajusz

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Imprinting was induced with synthetic oligopeptides in Chang liver cell cultures to test these molecules for signal molecule value. Investigations into imprinting overlaps (cross-imprinting) have shown that all oligopeptides (di-, tetra- and pentapeptides) carrying a terminal proline group were able to imprint the cells for the pentapeptide Tyr-D-Met-Gly-Phe-Pro-NH2, which displayed an outstanding imprinting potential for itself and an extraordinary opioid activity as well. The fact that exclusively the proline-deficient oligopeptide (a tetrapeptide) failed to imprint for the peptapeptide in question, indicates a decisive role of proline in the transformation of molecules to signal carriers (hormones). The pentapeptide in question did imprint for the related molecules (except the dipeptide) but to a much lesser degree than for itself. The marked inferiority of the pentapeptide's cross-imprinting potential to its self-imprinting potential supports the hypothetical implication that a considerable difference between the specific and non-specific binding capacities of a molecule, if not the loss of non-specific binding was an essential prerequisite of transformation to a signal molecule, i.e. of hormone evolution.

Original languageEnglish
Pages (from-to)231-235
Number of pages5
JournalBioSystems
Volume20
Issue number3
DOIs
Publication statusPublished - 1987

Fingerprint

oligopeptides
Oligopeptides
imprinting
Hormones
Proline
Liver
hepatocytes
hormone
Overlapping
proline
hormones
Molecules
Cell
dipeptides
Dipeptides
narcotics
binding capacity
Opioid Analgesics
cell culture
Cell Culture Techniques

Keywords

  • Cell line
  • Hormonal imprinting
  • Hormone evolution
  • Oligopeptides

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Biotechnology
  • Drug Discovery

Cite this

Overlapping imprinting of oligopeptides in Chang liver cells. Data on the mechanism of hormone evolution. / Csaba, G.; Kovács, P.; Tóth, S.; Bajusz, S.

In: BioSystems, Vol. 20, No. 3, 1987, p. 231-235.

Research output: Contribution to journalArticle

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