Overexpression of the Arabidopsis glutathione peroxidase-like 5 gene (AtGPXL5) resulted in altered plant development and redox status

Riyazuddin Riyazuddin, Krisztina Bela, E. Horváth, Gábor Rigó, Ágnes Gallé, L. Szabados, A. Fehér, J. Csiszár

Research output: Contribution to journalArticle

Abstract

Plant's glutathione peroxidase-like (GPXL) enzymes are thiol-based peroxidases catalysing the reduction of H2O2 or hydroperoxides to water or alcohols using reduced glutathione (GSH) or thioredoxin as an electron donor. Arabidopsis thaliana possess eight isoenzymes having different roles in redox-dependent processes. AtGPXL5 is a poorly known plasma membrane associated enzyme. We have investigated the role of AtGPXL5 in development and responses to salt using AtGPXL5-overexpressing lines (OX-AtGPXL5) and Atgpxl5 mutants. Constitutive overexpression of AtGPXL5 increased the transcription of the gene by 17–24 times in 6-week-old plants. In OX-AtGPXL5 plants, the activity of glutathione peroxidase, thioredoxin peroxidase and most of the main antioxidant enzymes were like in the wild type Col-0, but the amount of GSH was increased, thus the redox potential became more negative compared to the wild type. The well-preserved germination rate, seedling growth and chlorophyll content of the OX-AtGPXL5 seedlings in the presence of 100 mM NaCl indicated the increased salt tolerance of AtGPXL5-overexpressing plants. In agreement, the Atgpxl5 knockdown mutants had enhanced salt stress sensitivity in comparison to the wild type. Our results indicate that AtGPXL5 may have function in the fine-tuning of ROS levels and redox status during salt stress.

Original languageEnglish
Article number103849
JournalEnvironmental and Experimental Botany
Volume167
DOIs
Publication statusPublished - Nov 1 2019

Fingerprint

glutathione peroxidase
plant development
Arabidopsis
salt
gene
enzyme
salt stress
genes
enzymes
seedling
peroxiredoxin
mutants
thiol
peroxidases
redox potential
hydroperoxides
thiols
salt tolerance
antioxidant
seedling growth

Keywords

  • Antioxidant mechanisms
  • Arabidopsis thaliana
  • Glutathione peroxidase-like
  • Reactive oxygen species
  • Redox homeostasis
  • Salt stress

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Agronomy and Crop Science
  • Plant Science

Cite this

Overexpression of the Arabidopsis glutathione peroxidase-like 5 gene (AtGPXL5) resulted in altered plant development and redox status. / Riyazuddin, Riyazuddin; Bela, Krisztina; Horváth, E.; Rigó, Gábor; Gallé, Ágnes; Szabados, L.; Fehér, A.; Csiszár, J.

In: Environmental and Experimental Botany, Vol. 167, 103849, 01.11.2019.

Research output: Contribution to journalArticle

@article{ae794ecf9a7c44419a0946494821785d,
title = "Overexpression of the Arabidopsis glutathione peroxidase-like 5 gene (AtGPXL5) resulted in altered plant development and redox status",
abstract = "Plant's glutathione peroxidase-like (GPXL) enzymes are thiol-based peroxidases catalysing the reduction of H2O2 or hydroperoxides to water or alcohols using reduced glutathione (GSH) or thioredoxin as an electron donor. Arabidopsis thaliana possess eight isoenzymes having different roles in redox-dependent processes. AtGPXL5 is a poorly known plasma membrane associated enzyme. We have investigated the role of AtGPXL5 in development and responses to salt using AtGPXL5-overexpressing lines (OX-AtGPXL5) and Atgpxl5 mutants. Constitutive overexpression of AtGPXL5 increased the transcription of the gene by 17–24 times in 6-week-old plants. In OX-AtGPXL5 plants, the activity of glutathione peroxidase, thioredoxin peroxidase and most of the main antioxidant enzymes were like in the wild type Col-0, but the amount of GSH was increased, thus the redox potential became more negative compared to the wild type. The well-preserved germination rate, seedling growth and chlorophyll content of the OX-AtGPXL5 seedlings in the presence of 100 mM NaCl indicated the increased salt tolerance of AtGPXL5-overexpressing plants. In agreement, the Atgpxl5 knockdown mutants had enhanced salt stress sensitivity in comparison to the wild type. Our results indicate that AtGPXL5 may have function in the fine-tuning of ROS levels and redox status during salt stress.",
keywords = "Antioxidant mechanisms, Arabidopsis thaliana, Glutathione peroxidase-like, Reactive oxygen species, Redox homeostasis, Salt stress",
author = "Riyazuddin Riyazuddin and Krisztina Bela and E. Horv{\'a}th and G{\'a}bor Rig{\'o} and {\'A}gnes Gall{\'e} and L. Szabados and A. Feh{\'e}r and J. Csisz{\'a}r",
year = "2019",
month = "11",
day = "1",
doi = "10.1016/j.envexpbot.2019.103849",
language = "English",
volume = "167",
journal = "Environmental and Experimental Botany",
issn = "0098-8472",
publisher = "Elsevier",

}

TY - JOUR

T1 - Overexpression of the Arabidopsis glutathione peroxidase-like 5 gene (AtGPXL5) resulted in altered plant development and redox status

AU - Riyazuddin, Riyazuddin

AU - Bela, Krisztina

AU - Horváth, E.

AU - Rigó, Gábor

AU - Gallé, Ágnes

AU - Szabados, L.

AU - Fehér, A.

AU - Csiszár, J.

PY - 2019/11/1

Y1 - 2019/11/1

N2 - Plant's glutathione peroxidase-like (GPXL) enzymes are thiol-based peroxidases catalysing the reduction of H2O2 or hydroperoxides to water or alcohols using reduced glutathione (GSH) or thioredoxin as an electron donor. Arabidopsis thaliana possess eight isoenzymes having different roles in redox-dependent processes. AtGPXL5 is a poorly known plasma membrane associated enzyme. We have investigated the role of AtGPXL5 in development and responses to salt using AtGPXL5-overexpressing lines (OX-AtGPXL5) and Atgpxl5 mutants. Constitutive overexpression of AtGPXL5 increased the transcription of the gene by 17–24 times in 6-week-old plants. In OX-AtGPXL5 plants, the activity of glutathione peroxidase, thioredoxin peroxidase and most of the main antioxidant enzymes were like in the wild type Col-0, but the amount of GSH was increased, thus the redox potential became more negative compared to the wild type. The well-preserved germination rate, seedling growth and chlorophyll content of the OX-AtGPXL5 seedlings in the presence of 100 mM NaCl indicated the increased salt tolerance of AtGPXL5-overexpressing plants. In agreement, the Atgpxl5 knockdown mutants had enhanced salt stress sensitivity in comparison to the wild type. Our results indicate that AtGPXL5 may have function in the fine-tuning of ROS levels and redox status during salt stress.

AB - Plant's glutathione peroxidase-like (GPXL) enzymes are thiol-based peroxidases catalysing the reduction of H2O2 or hydroperoxides to water or alcohols using reduced glutathione (GSH) or thioredoxin as an electron donor. Arabidopsis thaliana possess eight isoenzymes having different roles in redox-dependent processes. AtGPXL5 is a poorly known plasma membrane associated enzyme. We have investigated the role of AtGPXL5 in development and responses to salt using AtGPXL5-overexpressing lines (OX-AtGPXL5) and Atgpxl5 mutants. Constitutive overexpression of AtGPXL5 increased the transcription of the gene by 17–24 times in 6-week-old plants. In OX-AtGPXL5 plants, the activity of glutathione peroxidase, thioredoxin peroxidase and most of the main antioxidant enzymes were like in the wild type Col-0, but the amount of GSH was increased, thus the redox potential became more negative compared to the wild type. The well-preserved germination rate, seedling growth and chlorophyll content of the OX-AtGPXL5 seedlings in the presence of 100 mM NaCl indicated the increased salt tolerance of AtGPXL5-overexpressing plants. In agreement, the Atgpxl5 knockdown mutants had enhanced salt stress sensitivity in comparison to the wild type. Our results indicate that AtGPXL5 may have function in the fine-tuning of ROS levels and redox status during salt stress.

KW - Antioxidant mechanisms

KW - Arabidopsis thaliana

KW - Glutathione peroxidase-like

KW - Reactive oxygen species

KW - Redox homeostasis

KW - Salt stress

UR - http://www.scopus.com/inward/record.url?scp=85070915001&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85070915001&partnerID=8YFLogxK

U2 - 10.1016/j.envexpbot.2019.103849

DO - 10.1016/j.envexpbot.2019.103849

M3 - Article

VL - 167

JO - Environmental and Experimental Botany

JF - Environmental and Experimental Botany

SN - 0098-8472

M1 - 103849

ER -