Overexpression of placental tissue protein 17b/TIP47 in cervical dysplasias and cervical carcinoma

G. Than, T. Turóczy, B. Sümegi, N. G. Than, S. Bellyei, H. Bohn, G. Szekeres

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

In our previous Western- and Northern-blot investigations, high over-expression of placental protein 17b/TIP47 was detected in extracts of human cervical carcinoma tissues compared to normal conditions of the cervical tissue. PP17b serum levels were also elevated in untreated cervical carcinoma patients compared to healthy controls. In the present study, the expression pattern of PP17 proteins was investigated in various cervical dysplasias and in cervical carcinoma tissue specimens by the streptavidin-biotin immunoperoxidase technique using PP17-specific antiserum. In normal third-trimester human placentas, which served as positive controls, mainly cytoplasmic PP17 immunostaining of syncytiotrophoblasts and chorionic trophoblasts was observed. Normal human uterine cervical squamous and glandular epithelia were negative or weakly positive, while in low grade dysplasias (CIN I-II) only the cytoplasms of dysplastic cells were weakly positive or positive; in high grade dysplasias (CIN III / ISC) cytoplasms of the dysplastic cells were strongly positive. Normal and superficial cells in the differentiated zones were negative in all tissue specimens. In cases of invasive epithelial cervical carcinomas, small basal-type tumour cells were mostly negative whilst cells with squamous differentiation were strongly positive for PP17. Our hypotheses for this newly detected phenomenon are briefly discussed.

Original languageEnglish
Pages (from-to)639-642
Number of pages4
JournalAnticancer Research
Volume21
Issue number1 B
Publication statusPublished - 2001

Fingerprint

Uterine Cervical Dysplasia
Pregnancy Proteins
Carcinoma
Trophoblasts
Cytoplasm
Streptavidin
Third Pregnancy Trimester
Biotin
Immunoenzyme Techniques
Northern Blotting
Placenta
Immune Sera
Epithelium
Western Blotting
Epithelial Cells
Serum
Neoplasms
Proteins

Keywords

  • Cervical carcinoma
  • Cervical dysplasia
  • Overexpression
  • PP17b/TIP47

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Overexpression of placental tissue protein 17b/TIP47 in cervical dysplasias and cervical carcinoma. / Than, G.; Turóczy, T.; Sümegi, B.; Than, N. G.; Bellyei, S.; Bohn, H.; Szekeres, G.

In: Anticancer Research, Vol. 21, No. 1 B, 2001, p. 639-642.

Research output: Contribution to journalArticle

Than, G. ; Turóczy, T. ; Sümegi, B. ; Than, N. G. ; Bellyei, S. ; Bohn, H. ; Szekeres, G. / Overexpression of placental tissue protein 17b/TIP47 in cervical dysplasias and cervical carcinoma. In: Anticancer Research. 2001 ; Vol. 21, No. 1 B. pp. 639-642.
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