Overexpression of human apolipoprotein B-100 induces severe neurodegeneration in transgenic mice

Erika Bereczki, Gábor Bernat, T. Csont, P. Ferdinándy, Henning Scheich, M. Sántha

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Recent studies showed correlation between increased serum apolipoprotein B-100 (apoB-100) level and Alzheimer's disease. To reveal the possible role of apoB-100 in neurodegeneration, we analyzed the serum lipoprotein and cerebral protein profiles, amyloid plaque formation, apoptosis and brain morphology of transgenic mice overexpressing the human apoB-100 protein. Serum lipoprotein profile showed significant increase of the plasma triglyceride level, while no alteration in total cholesterol was detected. The antibody microarray experiment revealed upregulation of several cytoskeletal, neuronal proteins and proteins that belong to the mitogen activated protein kinase pathway, indicating active apoptosis in the brain. Histochemical experiments showed formation of amyloid plaques and extensive neuronal death. Biochemical changes severely affected brain morphology; a dramatic genotype-dependent enlargement of the third and lateral ventricles in the brain was detected. On the basis of earlier and present results, we conclude that overexpressed human apoB-100 protein significantly increases the level of serum lipids (triglyceride upon normal chow diet and cholesterol on cholesterol-rich diet) which leads to cerebrovascular lesions and subsequently induces apoptosis and neurodegeneration.

Original languageEnglish
Pages (from-to)2246-2252
Number of pages7
JournalJournal of Proteome Research
Volume7
Issue number6
DOIs
Publication statusPublished - Jun 2008

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Apolipoprotein B-100
Transgenic Mice
Brain
Cholesterol
Amyloid Plaques
Apoptosis
Nutrition
Serum
Lipoproteins
Triglycerides
Diet
Third Ventricle
Cytoskeletal Proteins
Lateral Ventricles
Microarrays
Mitogen-Activated Protein Kinases
Amyloid
Alzheimer Disease
Proteins
Up-Regulation

Keywords

  • Apolipoprotein b-100
  • Cerebral protein profile
  • Magnetic resonance imaging
  • Neurodegenerative disorders
  • Transgenic mice
  • Ventricle enlargement

ASJC Scopus subject areas

  • Biochemistry
  • Chemistry(all)
  • Genetics
  • Biotechnology

Cite this

Overexpression of human apolipoprotein B-100 induces severe neurodegeneration in transgenic mice. / Bereczki, Erika; Bernat, Gábor; Csont, T.; Ferdinándy, P.; Scheich, Henning; Sántha, M.

In: Journal of Proteome Research, Vol. 7, No. 6, 06.2008, p. 2246-2252.

Research output: Contribution to journalArticle

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AU - Sántha, M.

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