Overexpression of dynamin is induced by chronic stimulation of μ but not δ-opioid receptors: Relationships with μ-related morphine dependence

Florence Noble, Maria Szücs, Brigitte Kieffer, Bernard P. Roques

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34 Citations (Scopus)

Abstract

Several studies using selective opioid agonists or mice with a deletion of the μ-opioid receptor, have shown that morphine dependence is essentially due to chronic stimulation of μ- but not δ-opioid receptors. Because dependence is assumed to be related to persistent intracellular modifications, we have investigated modifications putatively induced by chronic activation of μ receptors with morphine or selective agonists in vitro in SH-SY5Y cells and in vivo in different strains of mice, including mice lacking the μ-opioid receptor gene. The results show a similar down- regulation and desensitization of μ and δ binding sites, whereas an overexpression of dynamin occurred only with μ agonists, strongly suggesting the relevance of this upregulation with the opiate dependence. Moreover, translocation of overexpressed dynamin from intracellular pools to plasma membranes was observed in chronic morphine-treated rats. This recruitment could be critically involved in long-lasting changes such as alterations of axonal transport observed in opioid dependence.

Original languageEnglish
Pages (from-to)159-166
Number of pages8
JournalMolecular pharmacology
Volume58
Issue number1
DOIs
Publication statusPublished - Jan 1 2000

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ASJC Scopus subject areas

  • Molecular Medicine
  • Pharmacology

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