Overexpression of biglycan in the heart of transgenic mice

An antibody microarray study

Erika Bereczki, Szilvia Gonda, T. Csont, Eva Korpos, A. Zvara, P. Ferdinándy, M. Sántha

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Biglycan, a small leucine rich proteoglycan, is expressed in almost every tissue of the body, mainly in the extracellular matrix of connective tissues. Although there is an increasing amount of data on the biological role of biglycan protein, its function is still poorly understood. We aimed to gather more information about the biological function of biglycan protein in the cardiac tissues, and its role in signal transduction. Therefore, we generated transgenic mice overexpressing the human biglycan protein and analyzed the cardiac protein profile of transgenic offsprings using quantitative real-time (QRT)-PCR and proteomics. QRT-PCR results showed that most members of extracellular matrix were downregulated whereas cadherins, TGF-β1, and TGF-β2 were upregulated. Antibody microarrayer experiment revealed that pyk2, RAF-1, Mcl-1, syntrophin, calmodulin, isoforms of NOS protein family (eNOS, nNOS, and iNOS), and synaptotagmin proteins were unambiguously upregulated in the heart of biglycan transgenic mice. In this study we show that biglycan directly or indirectly activates proteins involved in cardiac remodeling (TGF-β, pyk2), signal transduction (RAF-1, Mcl-1, syntrophin, calmodulin, nNOS p38MAPK and MAP kinases), cardioprotection (NOS family, TGF-β) and Ca++ signaling (connexin, calmodulin, synaptotagmin). On the basis of the results presented here, we conclude that biglycan is a multifunctional extracellular protein that has a pivotal role in pathological remodeling of cardiac tissue and mediates cardioprotection.

Original languageEnglish
Pages (from-to)854-861
Number of pages8
JournalJournal of Proteome Research
Volume6
Issue number2
DOIs
Publication statusPublished - Feb 2007

Fingerprint

Biglycan
Microarrays
Transgenic Mice
Antibodies
Calmodulin
Synaptotagmins
Proteins
Tissue
Signal transduction
Extracellular Matrix
Real-Time Polymerase Chain Reaction
Signal Transduction
Connexins
Cadherins
Connective Tissue
Proteomics
Proteoglycans
Leucine
Protein Isoforms
Phosphotransferases

Keywords

  • Antibody microarray
  • Biglycan
  • Cardiac protein profile
  • Transgenic mice

ASJC Scopus subject areas

  • Genetics
  • Biotechnology
  • Biochemistry

Cite this

Overexpression of biglycan in the heart of transgenic mice : An antibody microarray study. / Bereczki, Erika; Gonda, Szilvia; Csont, T.; Korpos, Eva; Zvara, A.; Ferdinándy, P.; Sántha, M.

In: Journal of Proteome Research, Vol. 6, No. 2, 02.2007, p. 854-861.

Research output: Contribution to journalArticle

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