Over-expression of the bovine FcRn in the mammary gland results in increased IgG levels in both milk and serum of transgenic mice

Wei Lu, Zhihui Zhao, Yaofeng Zhao, Shuyang Yu, Yiqiang Zhao, Baoliang Fan, I. Kacskovics, Lennart Hammarström, Ning Li

Research output: Contribution to journalArticle

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Abstract

The neonatal Fc receptor (FcRn) protects immunoglobulin G (IgG) from catabolism and is also responsible for IgG absorption in the neonatal small intestine. However, whether it mediates the transfer of IgG from plasma to milk still remains speculative. In the present study, we have generated transgenic mice that over-express the bovine FcRn (bFcRn) in their lactating mammary glands. Significantly increased IgG levels were observed in the sera and milk from transgenic animals, suggesting that the over-expressed bFcRn could bind and protect endogenous mouse IgG and thus extend its lifespan. We also found that injected human IgG showed a significantly longer half-life (7-8 days) in the transgenic mice than in controls (2.9 days). Altogether, the data suggested that bFcRn could bind both mouse and human IgG, showing a cross-species FcRn-IgG binding activity. However, we found no selective accumulation of endogenous mouse IgG or injected bovine IgG in the milk of the transgenic females, supporting a previous hypothesis that IgG was transported from serum to milk in an inverse correlation to its binding affinity to FcRn.

Original languageEnglish
Pages (from-to)401-408
Number of pages8
JournalImmunology
Volume122
Issue number3
DOIs
Publication statusPublished - Nov 2007

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Human Mammary Glands
Transgenic Mice
Milk
Immunoglobulin G
Serum
Genetically Modified Animals
Small Intestine
Half-Life

Keywords

  • Immunoglobulin G
  • Neonatal Fc receptor
  • Transgenic mice

ASJC Scopus subject areas

  • Immunology

Cite this

Over-expression of the bovine FcRn in the mammary gland results in increased IgG levels in both milk and serum of transgenic mice. / Lu, Wei; Zhao, Zhihui; Zhao, Yaofeng; Yu, Shuyang; Zhao, Yiqiang; Fan, Baoliang; Kacskovics, I.; Hammarström, Lennart; Li, Ning.

In: Immunology, Vol. 122, No. 3, 11.2007, p. 401-408.

Research output: Contribution to journalArticle

Lu, Wei ; Zhao, Zhihui ; Zhao, Yaofeng ; Yu, Shuyang ; Zhao, Yiqiang ; Fan, Baoliang ; Kacskovics, I. ; Hammarström, Lennart ; Li, Ning. / Over-expression of the bovine FcRn in the mammary gland results in increased IgG levels in both milk and serum of transgenic mice. In: Immunology. 2007 ; Vol. 122, No. 3. pp. 401-408.
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