Ovarian stimulation and ultrasound-guided oocyte retrieval in baboon (Papio cynocephalus anubis) during pituitary suppression with a GnRH agonist

S. Cseh, J. Corselli, P. Chan, L. Bailey

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The objective of this study was to investigate whether baboon females respond to an ovarian stimulation protocol incorporating pituitary suppression with a GnRH agonist (GnRHa) and either highly purified human FSH (hphFSH) or recombinant human FSH (rhFSH) with follicular development and oocyte maturation. A modified human ovulation induction protocol was applied to 5 adult female baboons with a history of regular menstrual cycles (33-34 days). A long-acting GnRHa implant containing goserelin acetate was placed subcutaneously (s.c.) on Days 22-24 of their menstrual cycle. Concentrations of serum oestradiol (E2), progesterone (P4) and human FSH were obtained by ELISA. Menses occurred ∼ 10 days after GnRHa implantation. Daily hphFSH or rhFSH (75 IU i.m.) treatments were started ∼ 10 days following menses. When the majority of follicles were ≥ 5 mm in diameter and the E2 levels had reached a maximum, hCG (2000 IU i.m.) was administered to induce final maturation of oocytes and ovulation. Thirty to 34 h after hCG administration, transabdominal follicular aspiration was performed using a variable frequency transvaginal transducer with ultrasound. A total of 71 oocytes were collected from 4 animals (average: 17). The meiotic maturity of oocytes was evaluated 3 h after retrieval. Ninety-one percent of oocytes were in metaphase 2 and of grades I and II which are appropriate for in vitro insemination.

Original languageEnglish
Pages (from-to)443-450
Number of pages8
JournalActa Veterinaria Hungarica
Volume49
Issue number4
DOIs
Publication statusPublished - 2001

Fingerprint

Papio cynocephalus
Papio anubis
Human Follicle Stimulating Hormone
Oocyte Retrieval
Ovulation Induction
Papio
Gonadotropin-Releasing Hormone
agonists
Oocytes
oocytes
Menstruation
menstruation
Menstrual Cycle
menstrual cycle
human chorionic gonadotropin
Goserelin
ovulation
Insemination
Metaphase
Ovulation

Keywords

  • Baboon
  • GnRH agonist
  • Ovarian stimulation
  • Ultrasound-guided oocyte retrieval

ASJC Scopus subject areas

  • veterinary(all)

Cite this

Ovarian stimulation and ultrasound-guided oocyte retrieval in baboon (Papio cynocephalus anubis) during pituitary suppression with a GnRH agonist. / Cseh, S.; Corselli, J.; Chan, P.; Bailey, L.

In: Acta Veterinaria Hungarica, Vol. 49, No. 4, 2001, p. 443-450.

Research output: Contribution to journalArticle

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