Osteopontin is down-regulated in hydatidiform mole

Jozsef Batorfi, Vilmos Fulop, Jae Hoon Kim, David R. Genest, Jozsef Doszpod, Samuel C. Mok, Ross S. Berkowitz

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Objective. Osteopontin (OPN) is a glycoprotein of the extracellular matrix that can bind to different types of receptors including integrins and CD44 receptors. Multiple binding affinity enables OPN to play a role in many physiological and pathological processes. OPN contributes to tumorigenesis in several types of cancers. OPN is also expressed by the endometrium and by trophoblast cells of the chorionic villus in human placenta, where OPN may regulate implantation and placentation in early pregnancies by promoting cell-cell interactions, adhesion, spreading, and migration of trophoblast. Our purpose was to determine the expression of OPN mRNA and protein in hydatidiform mole and in normal placenta of comparable gestational age. Methods. A total of 13 fresh tissues from complete hydatidiform moles, 2 from partial hydatidiform moles, and 9 from normal placentas were analyzed by performing quantitative real-time PCR on microdissected trophoblast cells and immunohistochemistry on frozen sections of tissue. Results. Our results showed significantly lower expression of OPN mRNA and protein in hydatidiform mole, and in particular complete mole (P = 0.001 by real-time PCR and P < 0.001 by immunohistochemistry) as compared to nermal placenta. Conclusion. Although precise molecular mechanisms of gestational trophoblastic diseases have not yet been determined, down-regulation of osteopontin may play an important role in the pathogenesis of molar pregnancy.

Original languageEnglish
Pages (from-to)134-139
Number of pages6
JournalGynecologic Oncology
Volume89
Issue number1
DOIs
Publication statusPublished - Apr 1 2003

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Keywords

  • Hydatidiform mole
  • Immunohistochemistry
  • LCM
  • Laser capture microdissection
  • OPN
  • Osteopontin
  • Placenta
  • Real-time PCR

ASJC Scopus subject areas

  • Oncology
  • Obstetrics and Gynaecology

Cite this

Batorfi, J., Fulop, V., Kim, J. H., Genest, D. R., Doszpod, J., Mok, S. C., & Berkowitz, R. S. (2003). Osteopontin is down-regulated in hydatidiform mole. Gynecologic Oncology, 89(1), 134-139. https://doi.org/10.1016/S0090-8258(03)00067-2