Origin of ATP release in the rat vas deferens: Concomitant measurement of [3H]noradrenaline and [14C]ATP

E. Vízi, Geoffrey Burnstock

Research output: Contribution to journalArticle

64 Citations (Scopus)

Abstract

High pressure liquid chromatography (HPLC) combined with radiochemical detection was used to show that [3H]noradrenaline (NA) and [14C]ATP were released concomitantly during field stimulation of the rat vas deferens. The release of radioactivity (3H and 14C) in response to three consecutive field stimulations was constant (S2/S1 and S3/S2: 0.89 ± 0.10 and 0.91 ± 0.04 for 3H and 1.03 ± 0.12 and 1.08 ± 0.07 for 14C, respectively) and Ca2+- dependent. Tetrodotoxin completely inhibited the release of both NA and ATP. However, the α1-antagonist, prazosin, enhanced the release of [3H]NA, and significantly reduced that of [14C]ATP. Exogenously applied NA produced an increase in ATP release which was antagonized by prazosin. 4-Aminopyridine substantially enhanced the release of ATP in the presence of prazosin, clearly indicating that some ATP originates from nerves. It is concluded that part of the ATP collected during simulation of the rat vas deferens was released from nerves together with NA. In addition, a significant amount of ATP was released secondarily as a result of NA action via α1-adrenoceptors. The experiments provide no information about whether the prazosin-sensitive component of release of ATP originates from smooth muscle or from axon terminals.

Original languageEnglish
Pages (from-to)69-77
Number of pages9
JournalEuropean Journal of Pharmacology
Volume158
Issue number1-2
DOIs
Publication statusPublished - Dec 6 1988

Fingerprint

Vas Deferens
Norepinephrine
Adenosine Triphosphate
Prazosin
4-Aminopyridine
Tetrodotoxin
Presynaptic Terminals
Adrenergic Receptors
Radioactivity
Smooth Muscle
High Pressure Liquid Chromatography

Keywords

  • ATP
  • Noradrenaline
  • Vas deferens
  • α-adrenoceptors

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience
  • Pharmacology

Cite this

Origin of ATP release in the rat vas deferens : Concomitant measurement of [3H]noradrenaline and [14C]ATP. / Vízi, E.; Burnstock, Geoffrey.

In: European Journal of Pharmacology, Vol. 158, No. 1-2, 06.12.1988, p. 69-77.

Research output: Contribution to journalArticle

@article{24166272e4854a20ad131b02048e6bf1,
title = "Origin of ATP release in the rat vas deferens: Concomitant measurement of [3H]noradrenaline and [14C]ATP",
abstract = "High pressure liquid chromatography (HPLC) combined with radiochemical detection was used to show that [3H]noradrenaline (NA) and [14C]ATP were released concomitantly during field stimulation of the rat vas deferens. The release of radioactivity (3H and 14C) in response to three consecutive field stimulations was constant (S2/S1 and S3/S2: 0.89 ± 0.10 and 0.91 ± 0.04 for 3H and 1.03 ± 0.12 and 1.08 ± 0.07 for 14C, respectively) and Ca2+- dependent. Tetrodotoxin completely inhibited the release of both NA and ATP. However, the α1-antagonist, prazosin, enhanced the release of [3H]NA, and significantly reduced that of [14C]ATP. Exogenously applied NA produced an increase in ATP release which was antagonized by prazosin. 4-Aminopyridine substantially enhanced the release of ATP in the presence of prazosin, clearly indicating that some ATP originates from nerves. It is concluded that part of the ATP collected during simulation of the rat vas deferens was released from nerves together with NA. In addition, a significant amount of ATP was released secondarily as a result of NA action via α1-adrenoceptors. The experiments provide no information about whether the prazosin-sensitive component of release of ATP originates from smooth muscle or from axon terminals.",
keywords = "ATP, Noradrenaline, Vas deferens, α-adrenoceptors",
author = "E. V{\'i}zi and Geoffrey Burnstock",
year = "1988",
month = "12",
day = "6",
doi = "10.1016/0014-2999(88)90254-3",
language = "English",
volume = "158",
pages = "69--77",
journal = "European Journal of Pharmacology",
issn = "0014-2999",
publisher = "Elsevier",
number = "1-2",

}

TY - JOUR

T1 - Origin of ATP release in the rat vas deferens

T2 - Concomitant measurement of [3H]noradrenaline and [14C]ATP

AU - Vízi, E.

AU - Burnstock, Geoffrey

PY - 1988/12/6

Y1 - 1988/12/6

N2 - High pressure liquid chromatography (HPLC) combined with radiochemical detection was used to show that [3H]noradrenaline (NA) and [14C]ATP were released concomitantly during field stimulation of the rat vas deferens. The release of radioactivity (3H and 14C) in response to three consecutive field stimulations was constant (S2/S1 and S3/S2: 0.89 ± 0.10 and 0.91 ± 0.04 for 3H and 1.03 ± 0.12 and 1.08 ± 0.07 for 14C, respectively) and Ca2+- dependent. Tetrodotoxin completely inhibited the release of both NA and ATP. However, the α1-antagonist, prazosin, enhanced the release of [3H]NA, and significantly reduced that of [14C]ATP. Exogenously applied NA produced an increase in ATP release which was antagonized by prazosin. 4-Aminopyridine substantially enhanced the release of ATP in the presence of prazosin, clearly indicating that some ATP originates from nerves. It is concluded that part of the ATP collected during simulation of the rat vas deferens was released from nerves together with NA. In addition, a significant amount of ATP was released secondarily as a result of NA action via α1-adrenoceptors. The experiments provide no information about whether the prazosin-sensitive component of release of ATP originates from smooth muscle or from axon terminals.

AB - High pressure liquid chromatography (HPLC) combined with radiochemical detection was used to show that [3H]noradrenaline (NA) and [14C]ATP were released concomitantly during field stimulation of the rat vas deferens. The release of radioactivity (3H and 14C) in response to three consecutive field stimulations was constant (S2/S1 and S3/S2: 0.89 ± 0.10 and 0.91 ± 0.04 for 3H and 1.03 ± 0.12 and 1.08 ± 0.07 for 14C, respectively) and Ca2+- dependent. Tetrodotoxin completely inhibited the release of both NA and ATP. However, the α1-antagonist, prazosin, enhanced the release of [3H]NA, and significantly reduced that of [14C]ATP. Exogenously applied NA produced an increase in ATP release which was antagonized by prazosin. 4-Aminopyridine substantially enhanced the release of ATP in the presence of prazosin, clearly indicating that some ATP originates from nerves. It is concluded that part of the ATP collected during simulation of the rat vas deferens was released from nerves together with NA. In addition, a significant amount of ATP was released secondarily as a result of NA action via α1-adrenoceptors. The experiments provide no information about whether the prazosin-sensitive component of release of ATP originates from smooth muscle or from axon terminals.

KW - ATP

KW - Noradrenaline

KW - Vas deferens

KW - α-adrenoceptors

UR - http://www.scopus.com/inward/record.url?scp=0024230608&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0024230608&partnerID=8YFLogxK

U2 - 10.1016/0014-2999(88)90254-3

DO - 10.1016/0014-2999(88)90254-3

M3 - Article

C2 - 2851460

AN - SCOPUS:0024230608

VL - 158

SP - 69

EP - 77

JO - European Journal of Pharmacology

JF - European Journal of Pharmacology

SN - 0014-2999

IS - 1-2

ER -