Oriented pattern of membrane-associated vesicles in fibroblasts

P. Röhlich, A. C. Allison

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

The topography of membrane-associated ("pinocytic" or "surface") vesicles was investigated with freeze-fracturing of cultured human (MRC-5) fibroblasts. The vesicles were found to have a tendency to form longitudinal rows in the direction of the long axis of the cell or its main cytoplasmic processes. Parallel rows often formed irregular areas leaving smooth membrane surfaces between them. Cells in both confluent and nonconfluent cultures showed a similar tendency of the vesicles to be arranged in longitudinal rows. This arrangement was partially disturbed by 10-5 and 10-6 M colchicine and was completely abolished by Cytochalasin B in concentrations of 5, 8, and 10 μg/ml. The linear orientation of the vesicles is thought to be induced by close association of oriented microfilament bundles with the plasma membrane. The patchy distribution of the vesicles on the membrane indicates that the membrane is heterogeneous in structure (dynamic mosaicism of the membrane).

Original languageEnglish
Pages (from-to)94-103
Number of pages10
JournalJournal of Ultrasructure Research
Volume57
Issue number1
DOIs
Publication statusPublished - 1976

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Fibroblasts
Membranes
Freeze Fracturing
Cytochalasin B
Mosaicism
Colchicine
Actin Cytoskeleton
Cell Membrane

ASJC Scopus subject areas

  • Molecular Biology
  • Anatomy

Cite this

Oriented pattern of membrane-associated vesicles in fibroblasts. / Röhlich, P.; Allison, A. C.

In: Journal of Ultrasructure Research, Vol. 57, No. 1, 1976, p. 94-103.

Research output: Contribution to journalArticle

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