Orientation of myelin proteolipid protein in the oligodendrocyte cell membrane

Judith M. Greer, Charissa A. Dyer, M. Pákáski, Cammie Symonowicz, Marjorie B. Lees

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

The orientation of proteins within a cell membrane can often be difficult to determine. A number of models have been proposed for the orientation of the myelin protein, proteolipid protein (PLP), each of which includes exposed domains on the intracellular and extracellular membrane faces. Immunolabeling experiments have localized the C-terminus and the region spanning amino acids 103-116 to the cytoplasmic face of the membrane, but no well characterized antibodies have been available that label extracellular PLP domains. In this report, we describe the generation and characterization of mouse monoclonal antibodies (mAb) against putative extramembrane domains. Three of the mAb, specific for PLP peptides 40-59, 178-191, or 215-232, immunostain live oligodendrocytes, indicating that these regions of the molecule are exposed on the external surface of the cell. In addition, we have used these mAb to study the time-course of incorporation of PLP into the oligodendrocyte membrane. These studies increase our knowledge of the orientation of PLP in the lipid bilayer and are relevant for understanding myelin function.

Original languageEnglish
Pages (from-to)431-440
Number of pages10
JournalNeurochemical Research
Volume21
Issue number4
DOIs
Publication statusPublished - Apr 1996

Fingerprint

Myelin Proteolipid Protein
Oligodendroglia
Proteolipids
Cell membranes
Cell Membrane
Monoclonal Antibodies
Proteins
Membranes
Intracellular Membranes
Lipid Bilayers
Myelin Sheath
Lipid bilayers
Labels
Amino Acids
Peptides
Antibodies
Molecules

Keywords

  • antibodies
  • extracellular domains
  • membrane orientation
  • myelin
  • oligodendrocytes
  • Proteolipid protein

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience
  • Biochemistry
  • Neuroscience(all)

Cite this

Orientation of myelin proteolipid protein in the oligodendrocyte cell membrane. / Greer, Judith M.; Dyer, Charissa A.; Pákáski, M.; Symonowicz, Cammie; Lees, Marjorie B.

In: Neurochemical Research, Vol. 21, No. 4, 04.1996, p. 431-440.

Research output: Contribution to journalArticle

Greer, Judith M. ; Dyer, Charissa A. ; Pákáski, M. ; Symonowicz, Cammie ; Lees, Marjorie B. / Orientation of myelin proteolipid protein in the oligodendrocyte cell membrane. In: Neurochemical Research. 1996 ; Vol. 21, No. 4. pp. 431-440.
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