Organization of the pigment molecules in the thylakoids and the chlorophyll a/c light-harvesting complex of a xanthophyte alga, Pleurochloris meiringensis. A linear dichroism study

Claudia Büchel, G. Garab

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

We have studied the orientation of the absorption transition dipoles of the pigment molecules in thylakoid membranes, in the isolated core complex of photosystem I (CCI) and in the chlorophyll a/c light-harvesting complex of photosystem II (Chl a/c LHC) of the xanthophyte alga Pleurochloris meiringensis. In thylakoids, linear dichroism (LD) spectra reveal two distinct orientations of the Q(y) transition dipoles of the Chl a molecules: the long-wavelength Chl a molecules tend to lie in the plane of the thylakoid membrane, while those at shorter wavelengths tilt out of the membrane plane. Our data show that in CCI, the orientation of Q(y) dipoles of Chl a, and the overall orientation pattern of all transition dipoles in the visible region, an very similar to those in higher-plant photosystem I particles. The Chl a molecules with their Q(y) dipoles tilting out of the membrane plane are associated with the LHC, and constitute a substantial part of the Chl a population. The majority of the carotenoid molecules of LHC also tend to tilt out of the membrane plane. These data further corroborate our earlier conclusion that the molecular organization of thylakoid membranes and most particularly of the Chl a/c LHC differs significantly from the organization of higher-plant thylakoids and the main Chl a/b complexes, respectively.

Original languageEnglish
Pages (from-to)199-204
Number of pages6
JournalJournal of Photochemistry and Photobiology, B: Biology
Volume44
Issue number3
DOIs
Publication statusPublished - Jul 31 1998

Fingerprint

algae
Thylakoids
light harvesting complex
Dichroism
chlorophylls
Chlorophyll
Algae
pigments
thylakoids
Pigments
dichroism
Photosystem I Protein Complex
membranes
photosystem I
Membranes
chlorophyll
Light
Molecules
dipoles
molecules

Keywords

  • Algae
  • Chlorophyll a/c light-harvesting complex
  • Linear dichroism
  • Photosynthesis

ASJC Scopus subject areas

  • Plant Science
  • Bioengineering
  • Physical and Theoretical Chemistry

Cite this

@article{5a652b459f5b4dc38ba1d86cccacd70a,
title = "Organization of the pigment molecules in the thylakoids and the chlorophyll a/c light-harvesting complex of a xanthophyte alga, Pleurochloris meiringensis. A linear dichroism study",
abstract = "We have studied the orientation of the absorption transition dipoles of the pigment molecules in thylakoid membranes, in the isolated core complex of photosystem I (CCI) and in the chlorophyll a/c light-harvesting complex of photosystem II (Chl a/c LHC) of the xanthophyte alga Pleurochloris meiringensis. In thylakoids, linear dichroism (LD) spectra reveal two distinct orientations of the Q(y) transition dipoles of the Chl a molecules: the long-wavelength Chl a molecules tend to lie in the plane of the thylakoid membrane, while those at shorter wavelengths tilt out of the membrane plane. Our data show that in CCI, the orientation of Q(y) dipoles of Chl a, and the overall orientation pattern of all transition dipoles in the visible region, an very similar to those in higher-plant photosystem I particles. The Chl a molecules with their Q(y) dipoles tilting out of the membrane plane are associated with the LHC, and constitute a substantial part of the Chl a population. The majority of the carotenoid molecules of LHC also tend to tilt out of the membrane plane. These data further corroborate our earlier conclusion that the molecular organization of thylakoid membranes and most particularly of the Chl a/c LHC differs significantly from the organization of higher-plant thylakoids and the main Chl a/b complexes, respectively.",
keywords = "Algae, Chlorophyll a/c light-harvesting complex, Linear dichroism, Photosynthesis",
author = "Claudia B{\"u}chel and G. Garab",
year = "1998",
month = "7",
day = "31",
doi = "10.1016/S1011-1344(98)00143-2",
language = "English",
volume = "44",
pages = "199--204",
journal = "Journal of Photochemistry and Photobiology B: Biology",
issn = "1011-1344",
publisher = "Elsevier",
number = "3",

}

TY - JOUR

T1 - Organization of the pigment molecules in the thylakoids and the chlorophyll a/c light-harvesting complex of a xanthophyte alga, Pleurochloris meiringensis. A linear dichroism study

AU - Büchel, Claudia

AU - Garab, G.

PY - 1998/7/31

Y1 - 1998/7/31

N2 - We have studied the orientation of the absorption transition dipoles of the pigment molecules in thylakoid membranes, in the isolated core complex of photosystem I (CCI) and in the chlorophyll a/c light-harvesting complex of photosystem II (Chl a/c LHC) of the xanthophyte alga Pleurochloris meiringensis. In thylakoids, linear dichroism (LD) spectra reveal two distinct orientations of the Q(y) transition dipoles of the Chl a molecules: the long-wavelength Chl a molecules tend to lie in the plane of the thylakoid membrane, while those at shorter wavelengths tilt out of the membrane plane. Our data show that in CCI, the orientation of Q(y) dipoles of Chl a, and the overall orientation pattern of all transition dipoles in the visible region, an very similar to those in higher-plant photosystem I particles. The Chl a molecules with their Q(y) dipoles tilting out of the membrane plane are associated with the LHC, and constitute a substantial part of the Chl a population. The majority of the carotenoid molecules of LHC also tend to tilt out of the membrane plane. These data further corroborate our earlier conclusion that the molecular organization of thylakoid membranes and most particularly of the Chl a/c LHC differs significantly from the organization of higher-plant thylakoids and the main Chl a/b complexes, respectively.

AB - We have studied the orientation of the absorption transition dipoles of the pigment molecules in thylakoid membranes, in the isolated core complex of photosystem I (CCI) and in the chlorophyll a/c light-harvesting complex of photosystem II (Chl a/c LHC) of the xanthophyte alga Pleurochloris meiringensis. In thylakoids, linear dichroism (LD) spectra reveal two distinct orientations of the Q(y) transition dipoles of the Chl a molecules: the long-wavelength Chl a molecules tend to lie in the plane of the thylakoid membrane, while those at shorter wavelengths tilt out of the membrane plane. Our data show that in CCI, the orientation of Q(y) dipoles of Chl a, and the overall orientation pattern of all transition dipoles in the visible region, an very similar to those in higher-plant photosystem I particles. The Chl a molecules with their Q(y) dipoles tilting out of the membrane plane are associated with the LHC, and constitute a substantial part of the Chl a population. The majority of the carotenoid molecules of LHC also tend to tilt out of the membrane plane. These data further corroborate our earlier conclusion that the molecular organization of thylakoid membranes and most particularly of the Chl a/c LHC differs significantly from the organization of higher-plant thylakoids and the main Chl a/b complexes, respectively.

KW - Algae

KW - Chlorophyll a/c light-harvesting complex

KW - Linear dichroism

KW - Photosynthesis

UR - http://www.scopus.com/inward/record.url?scp=0032584489&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0032584489&partnerID=8YFLogxK

U2 - 10.1016/S1011-1344(98)00143-2

DO - 10.1016/S1011-1344(98)00143-2

M3 - Article

VL - 44

SP - 199

EP - 204

JO - Journal of Photochemistry and Photobiology B: Biology

JF - Journal of Photochemistry and Photobiology B: Biology

SN - 1011-1344

IS - 3

ER -