Organization of the Arp2/3 complex in hippocampal spines

B. Rácz, Richard J. Weinberg

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

Changes in the morphology of a dendritic spine require remodeling of its actin-based cytoskeleton. Biochemical mechanisms underlying actin remodeling have been studied extensively, but little is known about the physical organization of the actin-binding proteins that mediate remodeling in spines. Long-term potentiation-inducing stimuli trigger expansion of the spine head, suggesting local extension and branching of actin filaments. Because filament branching requires the Arp2/3 complex, we used quantitative immunoelectron microscopy to elucidate the organization of ARPC-2 (Arp2/3 complex subunit 2), an essential component of the complex. Our data from CA1 hippocampus indicate that Arp2/3 concentrates within spines in a previously unrecognized torroidal domain, apparently specialized to mediate actin filament branching.

Original languageEnglish
Pages (from-to)5654-5659
Number of pages6
JournalJournal of Neuroscience
Volume28
Issue number22
DOIs
Publication statusPublished - May 28 2008

Fingerprint

Actin-Related Protein 2-3 Complex
Actin Cytoskeleton
Spine
Microfilament Proteins
Dendritic Spines
Immunoelectron Microscopy
Long-Term Potentiation
Actins
Hippocampus

Keywords

  • Actin
  • Cytoskeleton
  • Hippocampus
  • LTP
  • Pyramidal cell
  • Synaptic plasticity

ASJC Scopus subject areas

  • Neuroscience(all)
  • Medicine(all)

Cite this

Organization of the Arp2/3 complex in hippocampal spines. / Rácz, B.; Weinberg, Richard J.

In: Journal of Neuroscience, Vol. 28, No. 22, 28.05.2008, p. 5654-5659.

Research output: Contribution to journalArticle

Rácz, B. ; Weinberg, Richard J. / Organization of the Arp2/3 complex in hippocampal spines. In: Journal of Neuroscience. 2008 ; Vol. 28, No. 22. pp. 5654-5659.
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